We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 5

1.
Figure 3

Figure 3. From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay.

Overlap of detected probes. Probes detected as present across all eight samples per target preparation method are compared. WB IVT: IVT-based direct hybridization with total RNA, GR IVT: IVT-based direct hybridization with globin-reduced RNA, WB DAP+: whole-genome DAP+ DASL with total RNA, WB DAP-: whole-genome DAP- DASL with total RNA, and GR DAP+: whole-genome DAP+ DASL with globin-reduced RNA.

Mary E Winn, et al. BMC Genomics. 2011;12:412-412.
2.
Figure 5

Figure 5. From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay.

Sample relations as assessed by unsupervised hierarchical clustering. Dendrogram reflecting the clustering of the individual samples and the different sample preparation methods. The dendrogram was constructed using hierarchical clustering methods as implemented in the Bioconductor lumi package.

Mary E Winn, et al. BMC Genomics. 2011;12:412-412.
3.
Figure 1

Figure 1. From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay.

Flow diagram of study design. A PAXGene blood tube was collected from 8 individuals then frozen and stored for later processing. RNA was isolated and microarray targets prepared by one of five different methods: IVT-based direct hybridization with total RNA (WB IVT), IVT-based direct hybridization with globin-reduced RNA (GR IVT), whole-genome DAP+ DASL with total RNA (WB DAP+), whole-genome DAP- DASL with total RNA (WB DAP-), and whole-genome DAP+ DASL with globin-reduced RNA (GR DAP+).

Mary E Winn, et al. BMC Genomics. 2011;12:412-412.
4.
Figure 2

Figure 2. From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay.

Box plots of present calls. The number of detected probes (detection p-value < 0.05) per target preparation method are shown. The boxes represent the lower quartile through the upper quartile, while the whiskers extend to 1.5 times the interquartile range. A bold line denotes the median. WB IVT and GR IVT (n = 8). WB DASL+, WB DASL-, and GR DAP+ (n = 16).

Mary E Winn, et al. BMC Genomics. 2011;12:412-412.
5.
Figure 4

Figure 4. From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay.

Raw intensity scatter plots. Raw intensities for all probes (n = 24526) were compared for (A) whole blood RNA and globin reduced RNA with IVT, (B) whole blood RNA with DAP+ and whole blood RNA with DAP-, (C) whole blood RNA and globin reduced RNA with DAP+, and (D) whole blood RNA with DAP- and globin reduced RNA with DAP-. Correlations for sample 1 are depicted. Average correlations for paired WB IVT versus GR IVT, WB DAP+ versus WB DAP-, WB DAP+ versus GR DAP+, and WB DAP- versus GR DAP- samples are 0.955, 0.992, 0.976, and 0.979, respectively. All 8 hemoglobin genes assayed on Illumina BeadChip Human-Ref v3.0 are labelled: HBA2, HBB, HBD, HBE1, HBG1, HBG2, HBM, HBQ, and HBZ. GLOBINclear specifically targets only HBA2 and HBB for reduction.

Mary E Winn, et al. BMC Genomics. 2011;12:412-412.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk