Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 8

1.
Fig. 2.

Fig. 2. From: Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle.

Anterior otic markers are reduced in lia–/– homozygotes; posterior markers are expressed as normal. (A-H) In situ hybridisation to anterior otic markers. (I-L) In situ hybridisation to posterior otic markers (arrows and brackets highlight the posterior otic domains). A-H,K,L: Dorsal views; anterior to left, medial to top. I,J: Lateral views; anterior to left, dorsal to top. Scale bars: 50 μm.

Katherine L. Hammond, et al. Development. 2011 September 15;138(18):3977-3987.
2.
Fig. 5.

Fig. 5. From: Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle.

Anterior otic markers are duplicated and posterior otic markers lost in hsp70:fgf3 zebrafish embryos heat-shocked at 10S. (A-F) In situ hybridisation to anterior and posterior otic markers. (G-J) In situ hybridisation to neurod, which marks delaminating neuroblasts and nascent neurons of the statoacoustic ganglion. A-F,I,J: Dorsal views; anterior to left, medial to top. G,H: Lateral views; anterior to left, dorsal to top. Dots delineate the outline of the otic vesicle in I and J. Scale bars: 50 μm.

Katherine L. Hammond, et al. Development. 2011 September 15;138(18):3977-3987.
3.
Fig. 6.

Fig. 6. From: Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle.

Altering Fgf and Hh signalling in the embryo does not affect otic expression of ptc1 and pea3, respectively. (A-H) ptc1 expression in hsp70:fgf3 compared with wild-type sibling zebrafish embryos heat-shocked at 10S, and in 10 μM SU5402-treated embryos compared with control DMSO-treated embryos treated from 10 to 20S. (I-P) pea3 expression in otic vesicles of ptc1–/–;ptc2–/– and smo–/– embryos compared with siblings. (Q,R) ptc1 expression in smo–/– and ptc1–/–;ptc2–/–embryos. (S,T) pea3 expression in 10 μM SU5402-treated and in hsp70:fgf3 embryos heat-shocked at 10S. A-H,Q,R: Dorsal views; anterior to left, lateral to top. I-P,S,T: Lateral views; anterior to left, dorsal to top. Dotted lines delineate otic vesicle outlines. Bracket in K marks low-level pea3 expression, lost from 21S ptc1–/–;ptc2–/– embryos (bracket, L).

Katherine L. Hammond, et al. Development. 2011 September 15;138(18):3977-3987.
4.
Fig. 1.

Fig. 1. From: Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle.

Anterior otic character is reduced in lia–/– (fgf3–/–) homozygotes. (A-F) Live 72 hpf lia–/– and sibling (sib) zebrafish inner ears. (G-L) Confocal z-stacks of 84 hpf ears stained with FITC-phalloidin to mark sensory hair cells. (M) The anterior macula of a 5 dpf lia–/– embryo stained with anti-acetylated tubulin antibody (kinocilia; red) and FITC-phalloidin (stereocilia; green). (N,O) Typical polarity maps for wild-type maculae. (P) Hair cell polarity map obtained from the specimen shown in M. (Q,R) Polarity maps from two further lia–/– specimens. A,B,D,E,G,H,J,K: Lateral views; anterior to left, dorsal to top. A,D,G,J: Lateral focal plane. B,E,H,K: Medial focal plane. C,F,I,L: Dorsal views; anterior to left, medial to top. am, anterior macula; ao, anterior otolith; c, cristae; pm, posterior macula; po, posterior otolith. Asterisks indicate semicircular canal pillars. Scale bars: 50 μm.

Katherine L. Hammond, et al. Development. 2011 September 15;138(18):3977-3987.
5.
Fig. 8.

Fig. 8. From: Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle.

A three-step model for AP axial patterning in the zebrafish ear. (A) Step 1 (prior to 10S): Induction of a symmetrical otic placode and establishment of equipotential poles. Fgf is required as a placode-inducing factor. Step 2 (10-20S): Direct response of the poles to instructive anteriorising (Fgf, pink) and posteriorising (Hh, blue) factors, with expression of target genes in otic tissue. Step 3 (20S+): The poles of the ear act as intrinsic organising centres, with different anterior (A) and posterior (P) identities. Signalling within the otic vesicle (curved arrow) reinforces initial asymmetry. (B) In the absence of Fgf signalling from 10 to 20S, both poles acquire posterior identity, as both are under the influence of Hh signalling from midline tissues. (C) In transgenic hsp70:fgf3 embryos heat-shocked from 10 to 20S, Fgf signalling is activated in all cells, and both poles acquire anterior identity. The red (anterior) and dark blue (posterior) arrows indicate tissue polarity in each half of the otic vesicle.

Katherine L. Hammond, et al. Development. 2011 September 15;138(18):3977-3987.
6.
Fig. 7.

Fig. 7. From: Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle.

Treatment of smo–/– embryos with SU5402 from 10 to 20S leads to a loss of anterior and posterior otic character. Ears of smo–/– and sibling zebrafish embryos treated with 10 μM SU5402 and DMSO, respectively, from 10 to 20S. (A,B) Anti-acetylated tubulin antibody stain marking the kinocilia of the first-forming hair cells (arrows). (C-J) FITC-phalloidin stain marking cell outlines and stereociliary bundles of the sensory hair cells. (C-F) Views of the whole vesicle. (G,H) Medial views showing the maculae. (I,J) Lateral views showing the cristae. (K-N) Inner ears of live embryos. Arrows indicate otoliths. cp, semicircular canal projection tissue. (O-T) In situ hybridisation to anterior and posterior otic markers. Dots delineate the otic vesicle in O and P. O,P,S,T: Dorsal views; anterior to left, medial to top. A-N,Q,R: Lateral views; anterior to left, dorsal to top. Scale bars: 50 μm.

Katherine L. Hammond, et al. Development. 2011 September 15;138(18):3977-3987.
7.
Fig. 4.

Fig. 4. From: Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle.

Heat shock of hsp70:fgf3 embryos at 10S leads to a complete loss of posterior otic domains and a mirror image duplication of anterior otic structures. (A-C) Ears of live 68 hpf hsp70:fgf3 and control wild-type zebrafish embryos heat-shocked at 10S. (D-L) Confocal z-stacks of 4 dpf ears stained with FITC-phalloidin to mark sensory hair cells. (D-F) Lateral focal planes showing the maculae. (G-I) Medial focal planes showing the cristae. (J-L) Dorsal views. (M-O) In situ hybridisation to otx1. (P) The macula of an 86 hpf heat-shocked hsp70:fgf3 embryo stained with anti-acetylated tubulin antibody (kinocilia; red) and FITC-phalloidin (stereocilia; green). (Q) Hair cell polarity maps obtained from the maculae shown in P. A-I: Lateral views; anterior to left, dorsal to top. J-Q: Dorsal views; anterior to left, medial to top. am, anterior macula; ao, anterior otolith; c, cristae; pm, posterior macula; po, posterior otolith. Arrow in I marks the lateral crista, which is enlarged. Scale bars: 50 μm.

Katherine L. Hammond, et al. Development. 2011 September 15;138(18):3977-3987.
8.
Fig. 3.

Fig. 3. From: Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle.

Treatment with SU5402 from 10 to 20S leads to a complete loss of anterior otic structures and a mirror image duplication of posterior otic structures. (A-H) Live ears of 84 hpf zebrafish embryos treated with 10 μM SU5402 and DMSO-treated controls. (I-P) Confocal z-stacks of 84 hpf SU5402-treated and control ears stained with FITC-phalloidin to mark sensory hair cells. (I-L) Medial focal planes showing the maculae. (M-P) Lateral focal planes showing the cristae. (Q) The macula of an 86 hpf SU5402-treated embryo stained with anti-acetylated tubulin antibody (kinocilia; red) and FITC-phalloidin (stereocilia; green). (R) Hair cell polarity map obtained from the macula shown in Q. (S) Polarity map obtained from a further SU5402-treated specimen. (T-W) In situ hybridisation to the anterior otic markers pax5 (T,U) and hmx2 (V,W). (X-Z′) In situ hybridisation to the posterior otic markers fst1 (X,Y) and otx1 (Z,Z′). (A′,B′) In situ hybridisation to neurod, which marks the statoacoustic ganglion. (C′,D′) In situ hybridisation to atoh1a, which marks the first hair cells. A-P,X,Y: Lateral views; anterior to left, dorsal to top. T-W,Z-D′: Dorsal views; anterior to left, medial to top. am, anterior macula; ao, anterior otolith; c, cristae; pm, posterior macula; po, posterior otolith. Asterisks in A indicate semicircular canal pillars; arrow in C indicates reduced lateral semicircular canal pillar. Dots in Z-D′ delineate the otic vesicle. Scale bars: 50 μm.

Katherine L. Hammond, et al. Development. 2011 September 15;138(18):3977-3987.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk