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Results: 4

1.
Figure 1

Figure 1. From: Hypertonic Saline Inhibits Arachidonic Acid Priming of the Human Neutrophil Oxidase.

HTS inhibits neutrophil priming by arachidonic acid. PMNs were incubated for 5 minutes with either isotonic or HTS buffer, primed with buffer (control) or AA (5 μM) for 10 minutes, activated with 200 ng/ml PMA for 5 minutes, and the superoxide anion release measured. This figure is the average superoxide release of n=4. `*' and `π' denotes (p<0.05 ANOVA, Newman-Keuls) significant priming versus buffer-treated control and significant superoxide inhibition of HTS group versus its isotonic counterpart, respectively.

Luis Lee, et al. J Surg Res. ;174(1):24-28.
2.
Figure 2

Figure 2. From: Hypertonic Saline Inhibits Arachidonic Acid Priming of the Human Neutrophil Oxidase.

HTS inhibits neutrophil priming by LTB4. PMNs were incubated for 5 minutes with either isotonic or HTS buffer, primed with buffer (control) or 100 nM, 1 μM, and 10 μM LTB4 for 5 minutes, activated with 200 ng/ml PMA for 5 minutes, and the superoxide anion release measured. This figure is the average superoxide release from of n=4. `*' and `π' denotes (p<0.05 ANOVA, Newman-Keuls) significant priming versus buffer-treated control and significant superoxide attenuation of HTS group versus its isotonic counterpart, respectively.

Luis Lee, et al. J Surg Res. ;174(1):24-28.
3.
Figure 3

Figure 3. From: Hypertonic Saline Inhibits Arachidonic Acid Priming of the Human Neutrophil Oxidase.

HTS does not inhibit AA receptor binding. Changes in cytosolic calcium concentration were measured in indo-1 AM-loaded PMNs (1 × 106 cells/ml) in a dual-wavelength spectrofluorimeter in real time. PMNs were pretreated with isotonic or HTS buffer for 5 mins at 37°C and subsequently stimulated with AA (5 μM) at 30 seconds. Both isotonic or HTS group showed a spike a calcium burst, suggesting succesful activation of a GPCR via ligand-binding followed by release of G proteins to increase intracellular Ca2+. This figure represents the average increase in intracellular Ca2+ of two separate experiments.

Luis Lee, et al. J Surg Res. ;174(1):24-28.
4.
Figure 4

Figure 4. From: Hypertonic Saline Inhibits Arachidonic Acid Priming of the Human Neutrophil Oxidase.

HTS does not inhibit LTB4 receptor binding. Changes in cytosolic calcium concentration were measured in iIndo-1 AM-loaded PMNs (1 × 106 cells/ml) in a dual-wavelength spectrofluorimeter in real time. PMNs were pretreated with isotonic or HTS buffer for 5 mins at 37°C and subsequently stimulated with LTB4 (1 μM) at 30 seconds. Both isotonic or HTS group showed a spike a calcium burst, indicating succesful activation of the GPCRs BLT1 via ligand-binding followed by release of G proteins to increase intracellular Ca2+. This figure represents the average increase in intracellular Ca2+ of two separate experiments.

Luis Lee, et al. J Surg Res. ;174(1):24-28.

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