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1.
Figure 2

Figure 2. Intracellular Morulae of the New Ehrlichia Species in the ISE6 and RF/6A Cell Cultures. From: Emergence of a New Pathogenic Ehrlichia Species, Wisconsin and Minnesota, 2009.

Panel A shows the ISE6 cell line, and Panel B shows the RF/6A cell line. Morulae are indicated by arrows (Giemsa stain).

Bobbi S. Pritt, et al. N Engl J Med. ;365(5):422-429.
2.
Figure 1

Figure 1. Genetic Relationships between the New Ehrlichia Species and Related Bacteria. From: Emergence of a New Pathogenic Ehrlichia Species, Wisconsin and Minnesota, 2009.

The arrow to the right of each phylogenetic tree indicates the newly discovered ehrlichia species (called “Wisconsin”). Panel A shows the phylogeny based on the 16S ribosomal RNA gene (rrs), inferred with the use of the minimum-evolution method and with distances calculated by means of the Jukes–Cantor method as the number of base substitutions per site. Panel B shows the phylogeny based on the GroEL heat-shock protein operon gene (groEL), inferred with the use of the neighbor-joining method and with distances calculated by means of the Kimura two-parameter method as the number of base substitutions per site. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (of 1000 replicates) is shown to the left of each branch. The trees are drawn to scale, with branch lengths in the same units as those of the evolutionary distances (see scale bars) used to infer the phylogenetic tree. Positions containing gaps, missing data, and primer sequences were eliminated from the data set. A total of 1160 positions for rrs and 591 positions for groEL were analyzed. Phylogenetic analyses were conducted with Molecular Evolutionary Genetics Analysis software, version 4.0.13 The GenBank accession number is listed at the end of each isolate name.

Bobbi S. Pritt, et al. N Engl J Med. ;365(5):422-429.

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