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Results: 5

1.
Fig. 4

Fig. 4. From: Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave.

Ca2+ excitation of rat ventricular myocytes and differentiated PC-12 in response to acupuncture-induced acoustic waves. a Confocal microscopy image of Fura-2/AM-loaded ventricular myocytes under a ×20 objective lens. Acoustic waves are turned on within 1 min upon recording. Contraction pulses and increase of Ca2+ transients are detected after a certain period of delay. b Confocal microscopy image of Fura-2/AM-loaded differentiated PC-12 under a ×60 objective lens. Ca2+ oscillations of differentiated PC-12 are detected when exposed to acoustic waves

Geng Li, et al. Pflugers Arch. 2011 October;462(4):587-597.
2.
Fig. 1

Fig. 1. From: Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave.

Study of acupuncture mechanism based on acoustic wave signaling. a Location of acupoint GB 35 in the gallbladder channel. T1 images showing the slice orientation of the MRE scan. b Coronal T1-weighted images, wave images in transverse direction, and wave images in longitudinal direction with needle at acupoint (blue open circle, 13), and adjacent non-acupoint (red open circle, 46). c Wave propagation profiles in longitudinal and transverse direction at acupoint. d Wave propagation profiles in longitudinal and transverse directions at non-acupoint

Geng Li, et al. Pflugers Arch. 2011 October;462(4):587-597.
3.
Fig. 5

Fig. 5. From: Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave.

In vivo Ca2+ signals induced by acupuncture in mouse skeletal muscle. a Transfected muscle fibers exhibited weak GCaMP2 fluorescence in vivo without ASW. b Enhanced brightness in fluorescence signal of GCaMP2 observed under ASW stimulation indicates cytosolic Ca2+ was excited by acupuncture. c Gd3+ completely reversed acupuncture-induced increase in plasma β-endorphin of the mice. Error bars represent SEM; n = 4; *p < 0.05 versus the acupuncture-treated group

Geng Li, et al. Pflugers Arch. 2011 October;462(4):587-597.
4.
Fig. 3

Fig. 3. From: Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave.

Ca2+ oscillations when endothelial H5V cells are exposed to ASW propagating in high-viscosity methyl cellulose solution. a Three typical profiles of single-cell Ca2+ recording. b Memory effects of H5V. c Statistical analysis of the dose-dependent effect of single-cell Ca2+ oscillations. The Ca2+ oscillation frequency and peak Ca2+ amplitude decrease as the acoustic wave attenuates with distance (0.5, 1, and 1.5 cm from the acoustic source, n = 8 × 3 = 24). And the single-cell Ca2+ response delay increases as the acoustic wave diminishes in the medium. *p < 0.05, significant versus the 0.5-cm group; #p < 0.05, significant versus the 1-cm group. d Response of H5V subjected to acoustic wave of different frequencies; region of interest around 0.5 cm away from the needle driver driven by a constant voltage

Geng Li, et al. Pflugers Arch. 2011 October;462(4):587-597.
5.
Fig. 2

Fig. 2. From: Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave.

Ca2+ excitation in fibroblast (NIH 3T3) by acupuncture-induced acoustic waves. a Piezoelectric driver to provide vertical motion of the needle inside methyl cellulose solution. b MRE wave images generated by the needle shear wave driver. c Simulated acoustic pressure distribution of methyl cellulose solution. COMSOL Multiphysics 3.4 was used to simulate the coupling of the needle shear wave to methyl cellulose. The maximum acoustic pressure is 3.457 Pa at the center. d Confocal microscopy image of Calcium Green-1-loaded NIH 3T3 cells under a ×10 objective lens. Peak Ca2+ transient spread from cells at the upper left then propagate to neighboring cells seconds later. e The 3D color-coded map shows the spatial distribution of latency of propagating Ca2+. The latency mesh is taken as the average of six individual experiments. f A 3D contour plot shows the spatial distribution of normalized peak amplitudes of Ca2+ transients. The amplitude mesh is taken as the average of six individual experiments. The product of the peak amplitude square and the latency is calculated to be constant of ∼13 at points 1 (0.52 × 0.52 × 45 = 12.2), 2 (0.35 × 0.35 × 100 = 12.3), and 3 (0.23 × 0.23 × 230 = 12.1)

Geng Li, et al. Pflugers Arch. 2011 October;462(4):587-597.

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