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Results: 4

1.
Fig. 2.

Fig. 2. From: Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors .

Neutralization profile of monoclonal antibodies CH01 to CH04. The neutralizing activity of MAbs CH01 to CH04 was tested on a panel of 91 pseudotyped lentiviruses, which comprised tier 1 and tier 2 isolates as well as transmitted founder viruses from multiple clades. Results are shown as IC50s (μg/ml) and color coded as shown in the key.

Mattia Bonsignori, et al. J Virol. 2011 October;85(19):9998-10009.
2.
Fig. 4.

Fig. 4. From: Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors .

Neutralizing activity of the putative reverted unmutated ancestor antibody candidates of the CH01 to CH04 clonal family antibodies. Two putative reverted ancestor antibody sequences (0219-RUA1 and 0219-RUA2) common to MAbs CH01 to CH04 were inferred and expressed as described in Materials and Methods. The two RUAs differed by a single nonsynonymous nucleotide mutation. The breadth of neutralization of 0219-RUA1 and 0219-RUA2 was tested against a panel of 24 pseudotyped lentiviruses comprising tier 1 and tier 2 isolates. Results are shown as IC50s (μg/ml) and color coded as shown in the key.

Mattia Bonsignori, et al. J Virol. 2011 October;85(19):9998-10009.
3.
Fig. 3.

Fig. 3. From: Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors .

Determination of the dissociation constant (Kd) from the gp120 E.A244 envelope glycoprotein by surface plasmon resonance (SPR) analysis. Serial dilutions ranging from 100 to 10 μg/ml of MAbs CH01 to CH04 (A to D, respectively), PG9 (E), PG16 (F), and putative reverted unmutated ancestor antibodies of the clonal lineage of CH01 to CH04 (G and H) were tested by SPR to identify the dissociation constants from the gp120 E.A244 envelope glycoprotein. The global curve-fitting χ2 values were between 1.9 and 0.08. The data shown are representative of duplicate experiments.

Mattia Bonsignori, et al. J Virol. 2011 October;85(19):9998-10009.
4.
Fig. 1.

Fig. 1. From: Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors .

Neutralization screening of primary IgG+ memory B cell cultures. IgG+ memory B cells were isolated from the peripheral blood of a broad neutralizer African subject chronically infected with a clade A HIV-1 strain. IgG+ memory B cells were cultured at a density of 8 cells/well in 3,600 culture wells for 14 days as described in Materials and Methods. At the end of stimulation, crude supernatants were tested for neutralizing activity against reporter tier 2 HIV-1 clade C isolate CAP45, a difficult-to-neutralize virus that was effectively neutralized by this subject's serum (Tomaras et al., unpublished). Solid dots represent the percentage of neutralization of each of the 3,600 cultures. Monoclonal antibodies CH01 to CH04 were isolated from the cultures represented by circled red dots. Positive controls (HIV Ig) are shown as open circles on the far right.

Mattia Bonsignori, et al. J Virol. 2011 October;85(19):9998-10009.

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