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1.
Fig. 6.

Fig. 6. From: CD11c Controls Herpes Simplex Virus 1 Responses To Limit Virus Replication during Primary Infection .

Effect of CD11c on interferon mRNAs in corneas and TG of CD11c-deficient mice. Total RNA was isolated from corneas and TG of infected mice on days 2, 4, and 6 p.i. and used for quantitative reverse transcription-PCR. IFN-α, IFN-β, and IFN-γ mRNA levels in naive mice were used to estimate the relative expression of each transcript in treated mice. GAPDH mRNA levels were used to normalize the relative expression of each transcript in corneas and TG of infected mice. Each point represents the mean ± SEM from 8 corneas or TG per time point.

Sariah J. Allen, et al. J Virol. 2011 October;85(19):9945-9955.
2.
Fig. 1.

Fig. 1. From: CD11c Controls Herpes Simplex Virus 1 Responses To Limit Virus Replication during Primary Infection .

Virus titers in eyes and TG following ocular infection of mice. (A) Ocular viral titers. CD11c−/− and wt mice were infected ocularly, and the amount of infectious HSV-1 in tear films was determined daily by standard plaque assays as described in Materials and Methods. For each time point, the virus titer (y axis) represents the average of the titers from 40 eyes ± SEM. (B) TG viral titers. CD11c−/− and wt mice were infected ocularly, and the amount of infectious HSV-1 in TG was determined on days 3 and 5 p.i. as described in Materials and Methods. For each time point, the virus titer (y axis) represents the average of the titers from 10 TG per time point ± SEM.

Sariah J. Allen, et al. J Virol. 2011 October;85(19):9945-9955.
3.
Fig. 2.

Fig. 2. From: CD11c Controls Herpes Simplex Virus 1 Responses To Limit Virus Replication during Primary Infection .

Elevated CD8, but not CD4, mRNA levels in corneas of CD11c-deficient mice. CD11c−/− and wt mice were infected ocularly as described in the legend to . Individual corneas from infected mice were isolated on days 2, 4, and 6 p.i. Total RNA was extracted, and cDNA was synthesized. Transcript expression in naive mice was used to estimate the relative expression of each transcript in corneas of infected mice. GAPDH expression was used to normalize the relative expression of each transcript in corneas of infected mice. Each point represents the mean ± SEM from 8 corneas per time point. (A) CD4; (B) CD8.

Sariah J. Allen, et al. J Virol. 2011 October;85(19):9945-9955.
4.
Fig. 4.

Fig. 4. From: CD11c Controls Herpes Simplex Virus 1 Responses To Limit Virus Replication during Primary Infection .

Effect of CD11c on T cells in TG of CD11c-deficient mice. CD11c-deficient and wt mice were infected as described in the legend to . On day 10 p.i., TG from 4 mice per group were homogenized individually and stained with anti-CD3, anti-CD8, and anti-HSV gB+498-505 pentamer antibodies as described in Materials and Methods. (A) Representative dot plots. Dot plots of T cells that are CD3+ CD8+ (left) and CD3+ CD8+ gB+498-505 pentamer (right) are shown. The number of positive cells in each quadrant is indicated at the upper right. (B) Quantification of CD8+ T cells. The mean numbers of CD3+ CD8+ (left) and CD3+ CD8+ gB+ (right) T cells per individual TG in CD11c-deficient and wt mice are shown. Each point represents the mean of 4 FACS analyses ± SEM.

Sariah J. Allen, et al. J Virol. 2011 October;85(19):9945-9955.
5.
Fig. 5.

Fig. 5. From: CD11c Controls Herpes Simplex Virus 1 Responses To Limit Virus Replication during Primary Infection .

Effect of CD11c on various responses in spleens of CD11c-deficient mice. CD11c-deficient and wt mice were infected as described in the legend to . On day 5 p.i., spleens from individual mice were homogenized and stained with antibodies specific for CD11b, HSV gB+498-505 pentamer, and CD83. (A) Representative dot plots. Dot plots from CD11b-positive (left), gB+498-505 pentamer-positive (center), and CD83-positive (right) cells. The number of positive cells is indicated in the upper left corner of each panel. (B) Quantification of the mean number of CD83+ (right), gB+498-505 pentamer (gB+; center), and CD11b+ (left) cells per individual spleen in CD11c-deficient and wt mice. The experiment was repeated 2 times for a total of 4 spleens ± SEM.

Sariah J. Allen, et al. J Virol. 2011 October;85(19):9945-9955.
6.
Fig. 3.

Fig. 3. From: CD11c Controls Herpes Simplex Virus 1 Responses To Limit Virus Replication during Primary Infection .

Effect of CD11c on CD8-positive T cells in corneas of ocularly infected mice. CD11c−/− and wt mice were infected ocularly as described in the legend to . (A) Qualitative analysis. Eyes from infected mice were isolated on days 5 and 10 p.i., fixed in OCT, sectioned, and stained for the presence of CD8+ T cells (green) as described in Materials and Methods. Cells were costained with DAPI (blue) to identify nuclei. Each individual panel shows CD8 staining alone or merge of CD8 and DAPI staining. Representative results are shown. (B) Quantitative analysis. The number of CD8+ T cells from the entire corneal section was counted in a double-blind fashion and plotted. Numbers shown represent the mean ± SEM from 18 CD11c-deficient corneal sections and 20 wt corneal sections from five mice per group.

Sariah J. Allen, et al. J Virol. 2011 October;85(19):9945-9955.
7.
Fig. 7.

Fig. 7. From: CD11c Controls Herpes Simplex Virus 1 Responses To Limit Virus Replication during Primary Infection .

Degree of CS and level of latency in infected mice. (A) CS. CS in surviving mice was determined 30 days after ocular infection. For each bar, CS (y axis) represents the average of 40 eyes from 4 separate experiments. (B) Latency. TG from infected mice used for measuring CS were removed individually at autopsy on day 30 p.i. TaqMan PCR of total DNA isolated from TG from each mouse was performed as described in Materials and Methods using gB primers. GAPDH DNA was used to normalize the relative expression of gB DNA in TG. In each experiment, an estimated relative copy number of gB was calculated using standard curves generated from pGem-gB1. Briefly, DNA template was serially diluted 10-fold, such that 5 μl contained from 103 to 1011 copies of gB, and then subjected to TaqMan PCR with the same set of primers. By comparing the normalized threshold cycle of each sample to the threshold cycle of the standard, the copy number for each reaction was determined. Each bar represents the mean ± SEM of 40 TG.

Sariah J. Allen, et al. J Virol. 2011 October;85(19):9945-9955.

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