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Results: 4

1.
Figure 4

Figure 4. qPCR analysis of gene expression after 18 lentectomies.. From: Regenerative capacity in newts is not altered by repeated regeneration and ageing.

Four different sets of lenses (from four different animals) were used and RNA was prepared separately from each. qPCR analysis was performed for each sample and the data combined, as shown in figure. C, control groups; E, experimental, no. 18. (a) qPCR data were normalized to housekeeping gene, GAPDH. (b) qPCR data were normalized to housekeeping gene EF1-a. Bars indicate the standard error of the mean (s.e.m.). Statistical analysis by t-test.

Goro Eguchi, et al. Nat Commun. 2011 July;2:384.
2.
Figure 3

Figure 3. qPCR analysis of gene expression after 17 lentectomies.. From: Regenerative capacity in newts is not altered by repeated regeneration and ageing.

Four different sets of lenses (from four different animals) were used and RNA was prepared separately from each. qPCR analysis was performed for each sample and the data combined, as shown in figure. C, control groups; E, experimental, no. 17. (a) qPCR data were normalized to housekeeping gene, GAPDH. (b) qPCR data were normalized to housekeeping gene EF1-a. Bars indicate the standard error of the mean (s.e.m.). Statistical analysis by t-test.

Goro Eguchi, et al. Nat Commun. 2011 July;2:384.
3.
Figure 2

Figure 2. Size of experimental and control regenerated lenses.. From: Regenerative capacity in newts is not altered by repeated regeneration and ageing.

Samples of regenerated lenses after the 17th (experimental no. 17) or the 18th (experimental no. 18) lentectomy compared with respective controls. Two samples from the eighteenth regeneration labelled as sample A and B are shown. Controls were intact lenses from animals that had never undergone regeneration. Images include a scale bar (a ruler with a basic unit of 1 mm) to indicate similarity in size of experimental and control lenses.

Goro Eguchi, et al. Nat Commun. 2011 July;2:384.
4.
Figure 1

Figure 1. Images of regenerated lenses after the seventeenth lentectomy.. From: Regenerative capacity in newts is not altered by repeated regeneration and ageing.

An experimental lentectomy no. 17 lens (right) compared with a control intact lens taken from an animal that had never undergone lens regeneration (control no. 17) (left). Top panels: photography of the whole lens (back lit or back and front lit). Note similarity in size and transparency as well as the normal arrangement of lens fibres around the equatorial circumference. Lower panels: sections from these lenses indicating a normal arrangement of lens fibres and normal lens morphology. From left to right: section showing lens fibre arrangement; a cross-section showing normal morphology of the lens with its nucleus (n) containing the primary fibres and the cortex (c) containing secondary fibres; staining of a section with β5-crystallin antibody to visualize fibres at higher magnification. Bar, 0.25 mm.

Goro Eguchi, et al. Nat Commun. 2011 July;2:384.

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