Results: 5

1.
Figure 2

Figure 2. From: Use of a novel virus detection assay to identify coronavirus HKU1 in the lungs of a hematopoietic stem cell transplant recipient with fatal pneumonia.

Lung biopsy. Hematoxylin and eosin staining of right lung biopsy obtained at week 15 post-transplant.

Christine Uhlenhaut, et al. Transpl Infect Dis. ;14(1):79-85.
2.

Figure 5. From: Use of a novel virus detection assay to identify coronavirus HKU1 in the lungs of a hematopoietic stem cell transplant recipient with fatal pneumonia.

Electron microscopy showing viral particles. Electron micrographs were performed on tissue obtained at lung biopsy at week 15 (A, 11,500X) and at autopsy at week 27 (B, 15,500X).

Christine Uhlenhaut, et al. Transpl Infect Dis. ;14(1):79-85.
3.
Figure 1

Figure 1. From: Use of a novel virus detection assay to identify coronavirus HKU1 in the lungs of a hematopoietic stem cell transplant recipient with fatal pneumonia.

Hospital course. Critical events during the patient’s hospitalizations are indicated, including use of antiviral and cytotoxic drugs. Cultures obtained at lung biopsy, BAL, and NP (nasopharyngeal) wash are denoted by circles. Closed circles represent positive cultures for influenza A virus, while open circles represent negative cultures.

Christine Uhlenhaut, et al. Transpl Infect Dis. ;14(1):79-85.
4.
Figure 3

Figure 3. From: Use of a novel virus detection assay to identify coronavirus HKU1 in the lungs of a hematopoietic stem cell transplant recipient with fatal pneumonia.

DOP-PCR results. Results of DOP-PCR on lung biopsy and lung tissue at autopsy are shown. Arrowheads indicate bands that were excised and cloned. The “X” marks the location of the band that yielded human coronavirus HKU1 sequence. RT- is the DOP-PCR reaction performed without a prior reverse transcriptase reaction. M=molecular weight marker, NTC= no template control

Christine Uhlenhaut, et al. Transpl Infect Dis. ;14(1):79-85.
5.
Figure 4

Figure 4. From: Use of a novel virus detection assay to identify coronavirus HKU1 in the lungs of a hematopoietic stem cell transplant recipient with fatal pneumonia.

Specific CoV-HKU1 PCR. Results of PCR on lung tissue obtained at autopsy to detect RNA sequences in the human coronavirus HKU1 ORF1ab gene (A), Spike-gene (B), or Nucleocapsid-gene (C), including reverse transcriptase-negative controls, are shown. (D) Genome organization and localization of PCR products. Locations relative to the human coronavirus HKU1 genome of DOP-PCR product (solid line) and of confirmatory PCR products (arrows) are shown. ORF1ab=open reading frame 1a and1b, HE=hemagglutinin-esterase, S=spike, E=envelope, M=membrane, N=nucleocapsid proteins of CoV-HKU1.

Christine Uhlenhaut, et al. Transpl Infect Dis. ;14(1):79-85.

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