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Results: 6

1.
Fig. 6.

Fig. 6. From: A key mechanism underlying sensory experience-dependent maturation of neocortical GABAergic circuits in vivo.

eIPSCs induced with graded stimulus intensities. (A) Left: Example of eIPSCs evoked by extracellular stimuli with graded intensity (from subthreshold to suprathreshold) in cells located in spared barrels and deprived barrels in layer IV at a Vhold of 0 mV. Right: Histogram of amplitudes of eIPSCs in respective recordings on the left. The distributions of amplitudes were fitted with multipeak Gaussian distribution. [a1–a4, values of center of the peak; solid lines, multipeak Gaussian fit.] (B) Scatter graph shows the value of mean Gaussian multi-peak distance (δa) in spared (filled circles) and deprived (open circles) cells.

Yuanyuan Jiao, et al. Proc Natl Acad Sci U S A. 2011 July 19;108(29):12131-12136.
2.
Fig. 5.

Fig. 5. From: A key mechanism underlying sensory experience-dependent maturation of neocortical GABAergic circuits in vivo.

Lack of sensory deprivation induced decrease sIPSCs in KIV−/−-GFP+/− mice. (A and B) Sample sIPSCs recorded in neurons from the spared and deprived region in KIV+/+ and KIV−/− mice at a Vhold of 0 mV. (C) Statistical graph for amplitudes, frequency, half-width, and decay time constant (τdecay). (A, ω-agatoxin-IVA 2 μM; dashed lines, controls.) (D) mIPSCs recorded in untreated control KIV+/+ and KIV−/− mice in the presence of TTX (Left). Histogram (Middle) of mIPSC amplitudes in representative cells and mean frequency of mIPSCs (Right; n = 10 cells in each group; P > 0.5, Kolmogorov–Smirnov test and one-way ANOVA, respectively).

Yuanyuan Jiao, et al. Proc Natl Acad Sci U S A. 2011 July 19;108(29):12131-12136.
3.
Fig. 4.

Fig. 4. From: A key mechanism underlying sensory experience-dependent maturation of neocortical GABAergic circuits in vivo.

Lack of effects of whisker trimming on ω-agatoxin–sensitive eIPSCs in KIV−/− mice. (A) FS-, but not RSNP neuron-, mediated IPSCs are selectively blocked by ω-agatoxin-IVA (2 μM). Left: Example of unitary IPSCs derived from a presynaptic FS cell in the absence (black trace) and presence (gray trace) of the toxin. Right: Example of unitary IPSPs derived from a presynaptic RSNP cell in the absence (black trace) and presence (gray trace) of the toxin (n = 4 pairs in each group). (B) Examples of recordings of eIPSCs induced with minimal stimulus in sensory spared (S) and deprived (D) area at a holding potential (Vhold) of 0 mV. (C) Mean amplitudes of eIPSCs, CV, and PPD in KIV+/+ versus KIV−/− mice, respectively (n = 15 in spared vs. n = 7 in deprived cortices).

Yuanyuan Jiao, et al. Proc Natl Acad Sci U S A. 2011 July 19;108(29):12131-12136.
4.
Fig. 2.

Fig. 2. From: A key mechanism underlying sensory experience-dependent maturation of neocortical GABAergic circuits in vivo.

Altered E/I balance in the KIV−/− mice. (A) Actual recording of postsynaptic current (Irecorded) for each genotype recorded under voltage-clamp recording with three holding potentials (−10, −40, −60 mV; left graph for each genotype). The responses were evoked by local stimulation under three stimulation intensities: the minimum (Top), 15% over the minimum (Middle), and the maximum (Bottom). Traces were averaged from 10 consecutive sweeps. The right graph for each genotype shows continuous plot of Gsyn (black), Ge (blue), and Gi (magenta) from layer IV spiny neurons at three different stimulus conditions in the KIV+/+ and KIV−/− mice, respectively. (BD) Mean E/I ratio (Ge/Gi) Ge, and Gi values induced by a range of stimulus conditions in KIV+/+ (black) and KIV−/− (red) mice, respectively (n = 10 neurons in each group).

Yuanyuan Jiao, et al. Proc Natl Acad Sci U S A. 2011 July 19;108(29):12131-12136.
5.
Fig. 3.

Fig. 3. From: A key mechanism underlying sensory experience-dependent maturation of neocortical GABAergic circuits in vivo.

Impairment in activity-dependent regulation of inhibitory perisomatic boutons. (A) Confocal micrographs of GFP and PV IR in layer IV barrel cortex. (Scale bars: 2 μm.) For each merged image, grayscale line profile of the entire image for PV (red) and GFP (green) and the cross-correlation (Corr) plot for the two line profile curves are shown on the right. Note that the expression of PV and GFP were highly overlapping (i.e., higher cross-correlation) in the KIV+/+ but not KIV−/− mice. (B) A neurofilament-positive (i.e., glutamatergic; red) neuron innervated by perisomatic GFP-positive (i.e., GABAergic; green) boutons. The number of GFP-positive perisomatic varicosities was normalized against the diameter of each presumed spiny neuron (Middle). (C) Bar graph shows the plot of mean number of perisomatic boutons per cell (normalized cell diameter), and cross-correlation (Right) in KIV+/+ and KIV−/− mice with whisker trimming manipulations (spared or deprived) and without (control) whisker trimming manipulations (n = 12 slices from four brains in each group).

Yuanyuan Jiao, et al. Proc Natl Acad Sci U S A. 2011 July 19;108(29):12131-12136.
6.
Fig. 1.

Fig. 1. From: A key mechanism underlying sensory experience-dependent maturation of neocortical GABAergic circuits in vivo.

Intact barrel structure with reduced expression of BDNF in the KIV−/− mice. (A) Vglut2 IR in a tangential section (Left) and CO histochemistry in a TC section (Right) show all barrels (rows A–E) intact in KIV−/− mice. (Scale bar: 150 μm.) Bottom: Line profile of grayscale intensity of vglut2-IR and CO across different rows shows the existence of barrels (marked with numbers 1–5 or letters A–E) and septum. (B) Photograph of BDNF IR and line profile of the photo in layer IV tangential section of barrel cortex (1× and 10× magnification, respectively). The intensity of BDNF IR was measured along the white lines across the barrel field and presented in the grayscale line profiles below. The grayscale line profile clearly shows existence of the barrel pattern in the KIV+/+ but not KIV−/− mice. Inset: Barrel field in KIV−/− mice was identified via similar region in an adjacent section stained with CO, which clearly indicates the barrel field, with a line profile below. (C) Photograph of BDNF IR and line profile of the photo (Lower) in TC sections across the barrel cortex. Bar graph (Right) shows the effect of all-row whisker trimming (n = 6 mice in each group) on the differences in grayscale intensities (ΔG) of BDNF IR between barrel and septum. (D) Regulation of BDNF levels by sensory inputs. Left: BDNF absorption levels measured with BDNF Emax ImmunoAssay System (Promega). Center: Example of Western blot showing proBDNF and mBDNF in lysate from KIV+/+ and KIV−/− mice, respectively. Purified proBDNF and mBDNF are shown in right lanes for comparison. Right: Quantification of the Western blots. Lysate was obtained from freshly cut barrel cortex from KIV+/+ and KIV−/− mice (n = 3 and n = 6 mice in each group, respectively). In this and all figures, one-way ANOVA was used (*P < 0.05, **P < 0.01, and ***P < 0.001).

Yuanyuan Jiao, et al. Proc Natl Acad Sci U S A. 2011 July 19;108(29):12131-12136.

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