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1.
Figure 1

Figure 1. From: The effect of oxidized low-density lipoprotein combined with adriamycin on the proliferation of Eca-109 cell line.

The viability of Eca-109 cells treated with oxLDL in the MTT test.

Hao Li, et al. Lipids Health Dis. 2011;10:108-108.
2.
Figure 3

Figure 3. From: The effect of oxidized low-density lipoprotein combined with adriamycin on the proliferation of Eca-109 cell line.

The frequencies of viability for the Eca-109 cell treated with oxLDL, ADM on alone or their combination. *: p < 0.05, **: p < 0.01 in ANOVA test.

Hao Li, et al. Lipids Health Dis. 2011;10:108-108.
3.
Figure 7

Figure 7. From: The effect of oxidized low-density lipoprotein combined with adriamycin on the proliferation of Eca-109 cell line.

The protein expression of Bcl-2, Bax and Caspase-3 genes for the Eca-109 cell treated with the oxLDL, ADM, and their combination for 48 h. β-actin serves as loading control. Note: line 1: control; line 2: 40 μg/ml oxLDL; line 3: 0.1 μg/ml ADM; Line 4: 40 μg/ml oxLDL + 0.1 μg/ml ADM. Abbreviation: ADM, adriamycin.

Hao Li, et al. Lipids Health Dis. 2011;10:108-108.
4.
Figure 2

Figure 2. From: The effect of oxidized low-density lipoprotein combined with adriamycin on the proliferation of Eca-109 cell line.

The frequencies of apoptosis for Eca-109 cells treated with three concentration (10, 40, 90 μg/ml) of oxLDL observed after 24 h, 48 h and 72 h of the treatment. *: Compared with the control, p < 0.05.

Hao Li, et al. Lipids Health Dis. 2011;10:108-108.
5.
Figure 6

Figure 6. From: The effect of oxidized low-density lipoprotein combined with adriamycin on the proliferation of Eca-109 cell line.

The RT-PCR expression of Bcl-2, Bax and Caspase-3 gene mRNA for the Eca-109 cell treated by the oxLDL, ADM, and both of them combined for 48 hours. β-actin serves as loading control. Note: line 1: DNA marker; line 2: control; line 3: 40 μg/ml oxLDL; line 4: 0.1 μg/ml ADM; Line 5: 40 μg/ml oxLDL + 0.1 μg/ml ADM. Abbreviation: ADM, adriamycin.

Hao Li, et al. Lipids Health Dis. 2011;10:108-108.
6.
Figure 4

Figure 4. From: The effect of oxidized low-density lipoprotein combined with adriamycin on the proliferation of Eca-109 cell line.

Morphology of Eca-109 cell apoptosis in light microscopy (magnification with x100 and x400, respectively for the same slice); When the Eca-109 cell treated with the drugs 48 h after the treatment, the morphology of the cell showed nuclear chromatin condensation and fragmentation, plasmic budding, phagocytosis of the extruded and apoptotic body. Note: The doses of drugs treated with 40 μg/ml oxLDL, 0.1 μg/ml ADM, and both of them combined.

Hao Li, et al. Lipids Health Dis. 2011;10:108-108.
7.
Figure 5

Figure 5. From: The effect of oxidized low-density lipoprotein combined with adriamycin on the proliferation of Eca-109 cell line.

A-1, B-1, C-1 and D-1 showed the distribution of percentages of apoptosis;, and A-2, B-2, C-2 and D-2 showed the distribution of percentages of cell cycle for Eca-109 cells treated with oxLDL or ADM alone and both of them combined after 48 h of the treatment. For cell cycle: A-2: 67.37 ± 5.49%, 22.03 ± 0.73% and 10.60 ± 0.26% in the G1/G0, S and G2/M phase of control; B-2: 59.45 ± 4.53%, 29.86 ± 1.77% and 10.69 ± 0.62% in the G1/G0, S and G2/M phase of the oxLDL; C-2: 24.12 ± 3.64%, 62.59 ± 4.29% and 13.28 ± 1.45% in the G1/G0, S and G2/M phase of the ADM; D-2: 8.73 ± 2.48%, 15.61 ± 5.17% and 75.66 ± 3.64% in the G1/G0, S and G2/M phase of the above oxLDL and ADM combined, respectively. The proportions of apoptosis and phase of cell cycle in 40 μg/ml oxLDL + 0.1 μg/ml ADM group were significant higher than that in other groups, p < 0.05. Dose: A-1 and A-2: control group; B-1 and B-2: 40 μg/ml oxLDL; C-1and C-2: 0.1 μg/ml ADM; D-1 and D-2: 40 μg/ml oxLDL + 0.1 μg/ml ADM. Abbreviation: ADM, adriamycin.

Hao Li, et al. Lipids Health Dis. 2011;10:108-108.

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