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1.
Figure 2

Figure 2. Phylogenetic analysis of virus genomes from LAPC4, VCaP and EKVX cell lines.. From: Identification of Replication Competent Murine Gammaretroviruses in Commonly Used Prostate Cancer Cell Lines.

The phylogenetic tree represents known xenotropic MLV sequences. This tree represents only xenotropic MLVs for which the full proviral sequence is known, which is likely only a small portion of all endogenous xenotropic MLVs present in mice.

Karen Sandell Sfanos, et al. PLoS One. 2011;6(6):e20874.
2.
Figure 1

Figure 1. LAPC4, VCaP and EKVX cancer cell lines are infected with a murine leukemia virus which is not XMRV.. From: Identification of Replication Competent Murine Gammaretroviruses in Commonly Used Prostate Cancer Cell Lines.

(A) Examples of MLV-negative (DU145) and positive (CWR22Rv1, LAPC4, VCaP and EKVX) cancer cell lines stained with MLV30 and MLV70 antisera. (B) Positive staining in LAPC4, VCaP and EKVX does not represent XMRV. Lanes 1–7, PCR with MLV-specific primers (1 =  CWR22Rv1, 2 =  LAPC4, 3 =  VCaP, 4 =  DU145, 5 =  LNCaP, 6 =  PC3, 7 =  EKVX), Lanes 8–11, PCR with XMRV-specific primers (8 =  CWR22Rv1, 9 =  LAPC4, 10 =  VCaP, 11 =  EKVX). Difference in product size between CWR22Rv1 (lane 1) and LAPC4, VCaP or EKVX (lanes 2,3,7) is due to the XMRV-specific 24-nt deletion [13]. L =  molecular weight ladder.

Karen Sandell Sfanos, et al. PLoS One. 2011;6(6):e20874.
3.
Figure 3

Figure 3. CWR22Rv1, LAPC4, VCaP and EKVX produce replication competent virus as indicated by Gaussia luciferase production in iGLuc-DERSE cells.. From: Identification of Replication Competent Murine Gammaretroviruses in Commonly Used Prostate Cancer Cell Lines.

10 µl of media was assayed 2 days after exposure to 24 hr. cell culture supernatant from CWR22Rv1, LAPC4 and VCaP, and 13 days (and 3 passages) after exposure to supernatant from EKVX, DU145, MycCaP (murine prostate cancer cell line) and PC3. Red line indicates background luciferase expression as determined by a mock-infected control. Error bars represent standard error of the mean for 3 experiments.

Karen Sandell Sfanos, et al. PLoS One. 2011;6(6):e20874.

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