Results: 3

Figure 1

Figure 1. From: MicroRNA-9.

Sequences and conservation of miR-9 and related miRNAs through evolution. (A) All vertebrate miR-9 sequences are all identical to insect miR-9a, displaying only the small differences in length at the 3′ end that are caused by differences in 3′ processing. Mature insect miR-9 paralogs, by contrast, often differ considerably in their middle and 3′ regions while maintaining orthology across species boundaries. (B) Stem-loop structures of Drosophila miR-9a and human miR-9-2 precursors. MiR-9a and miR-9 sequences are in red. The miR-9* sequence is in blue.

Yeliz Yuva-Aydemir, et al. RNA Biol. 2011 Jul-Aug;8(4):557-564.
Figure 2

Figure 2. From: MicroRNA-9.

Alignments were generated using the PRANK alignment package15 for miR-9 precursors in human, chimpanzee, gorilla, mouse, platypus, chicken, frog, zebrafish and fugu within vertebrates, and in fruit fly, mosquito and silkworm within invertebrates. Ggo-miR-9-1 (labeled with ‘#’) and Ggo-miR-9-3 (labeled with ‘&’) derive from annotations of the GorGor3 genome assembly. All other miRNAs derive directly from miRbase release 16.14 The evolutionary history was inferred using the maximum Parsimony method, and of several parsimonious trees, the tree most consistent with mature sequences as well as existing miR-9 nomenclature was chosen. The MP tree was obtained using the Close-Neighbor-Interchange algorithm (pg. 128 in ref. 16). Only tree topology is shown here for simplicity. Evolutionary analyses were conducted in MEGA5,17 and the length of each line does not reflect the relative evolutionary distance.

Yeliz Yuva-Aydemir, et al. RNA Biol. 2011 Jul-Aug;8(4):557-564.
Figure 3

Figure 3. From: MicroRNA-9.

MiR-9 expression during development in different species, as shown by in situ hybridization. (A) MiR-9a is expressed in ectoderm but not in the central nervous system in stage 12 Drosophila embryo.17 (B) In Drosophila wing imaginal disc, miR-9a (red) is not expressed in SOP cells (green) but is expressed in adjacent epithelial cells.18 (C) Lateral view of whole-mount zebrafish embryo shows prominent miR-9 expression in the nervous system at 48 h after fertilization.11 (D) Dorsal view of a zebrafish embryo (35 h after fertilization), miR-9 is detected in the midbrain and hindbrain (blue arrows) but absent in the MHB (arrowhead).19 Te, tectum; CB, cerebellar plate. Blue arrows indicate the midbrain and the hindbrain. (E) Xenopus miR-9 is mostly expressed in the anterior neural tube but not in the spinal cord (whole mount, dorsal view, stage 30). As in zebrafish, the MHB is devoid of miR-9 (red asterisk).20 Scale bar, 200 µm. (F) Transverse section of a stage 30 Xenopus embryo shows miR-9 expression in the proliferative ventricular zone.20 (G) In the chick embryo (HH20), miR-9 is found in the telencephalic vesicles (arrow), diencephalon (arrowhead) and spinal cord.21 (H) Section of developing chick spinal cord (HH24) demonstrated that miR-9 is present in both proliferating precursors in the ventricular zone and a subset of motor neurons (arrows).22 (I) Whole-mount mouse embryo (E10) shows miR-9 expression in the telencephalon (arrow) and hindbrain (arrowhead).23 (J) Section through the developing mouse forebrain (E12.5) shows no obvious overlap between Foxg1 (red) and miR-9 (purple) expression.23 All images are reproduced with permission.

Yeliz Yuva-Aydemir, et al. RNA Biol. 2011 Jul-Aug;8(4):557-564.

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