Results: 4

1.
Fig. 1.

Fig. 1. From: Analysis of Insertion Sequences in Thermophilic Cyanobacteria: Exploring the Mechanisms of Establishing, Maintaining, and Withstanding High Insertion Sequence Abundance .

ISSoc subfamily abundance in the metagenome. Reads were binned taxonomically by similarity to the reference Synechococcus OS-A and Synechococcus OS-B′ genomes. OS-like, reads met bin criteria for both references; other, reads met bin criteria for neither reference (see the text for further details).

William C. Nelson, et al. Appl Environ Microbiol. 2011 August;77(15):5458-5466.
2.
Fig. 2.

Fig. 2. From: Analysis of Insertion Sequences in Thermophilic Cyanobacteria: Exploring the Mechanisms of Establishing, Maintaining, and Withstanding High Insertion Sequence Abundance .

Phylogenetic analysis of the ISSoc1 subfamily does not show species-specific branching. All full-length copies of ISSoc1 from both Synechococcus OS-A and Synechococcus OS-B′ were used to create a rooted neighbor-joining tree, using the maximum composite likelihood model and 500 bootstrap replicates. Nodes with asterisks (*) denote ≥50% bootstrap support.

William C. Nelson, et al. Appl Environ Microbiol. 2011 August;77(15):5458-5466.
3.
Fig. 3.

Fig. 3. From: Analysis of Insertion Sequences in Thermophilic Cyanobacteria: Exploring the Mechanisms of Establishing, Maintaining, and Withstanding High Insertion Sequence Abundance .

Putative laterally transferred regions contain ISSoc sequences. The syntenic regions displayed (A, B, and C) are >93% identical (nucleotide identity). Numbers indicate genome coordinates. The OS-B′ region in panel C is presented reversed for ease of display. Genes in red indicate ISSoc transposase genes that are syntenic in both isolates. Genes in yellow indicate ISSoc transposase genes found in only one of the isolates. Other colors indicate genes with similar functional annotations and illustrate the overall synteny of the region. The figure was adapted from images generated by the IMG Chromosome Viewer (22).

William C. Nelson, et al. Appl Environ Microbiol. 2011 August;77(15):5458-5466.
4.
Fig. 4.

Fig. 4. From: Analysis of Insertion Sequences in Thermophilic Cyanobacteria: Exploring the Mechanisms of Establishing, Maintaining, and Withstanding High Insertion Sequence Abundance .

Evidence of ISSoc activity detected by comparative analysis. (A) Incongruous read analysis. Metagenome sequences with two (or more) nonoverlapping regions mapped to nonadjacent areas of the reference genome, and one region consisted entirely of ISSoc sequences. (B) Spanning read analysis. Metagenome sequences with two nonoverlapping regions mapped to nearly adjacent (≤2,000 nt) regions of the reference genome, and the intervening region between the map positions consisted entirely of ISSoc sequences. White bars, Synechococcus OS-A-like population; black bars, Synechococcus OS-B′-like population.

William C. Nelson, et al. Appl Environ Microbiol. 2011 August;77(15):5458-5466.

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