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Results: 5

1.
Figure 2

Figure 2. From: Casein kinase 1 enables nucleus accumbens amphetamine induced locomotion by regulating AMPA receptor phosphorylation.

PF blocks NAcc AMPH induced phosphorylation of Darpp-32. Representative immunoblots of (A) pDarpp-32(S137), Darpp-32 and Csnk1δ/ε and (D) pDarpp-32(T34) in the NAcc of rats in the four treatment groups. (B) pDarpp-32(S137)/Darpp-32 ratios (*, P<0.05, vs Saline; ††, P<0.01, vs AMPH; Scheffé), (C) Csnk1δ/ε protein levels, (E) pDarpp-32(T34)/Darpp-32 ratios (*, P<0.05, vs Saline; †, P<0.05, vs AMPH; Scheffé), and (F) Darpp-32 protein levels obtained in the NAcc for the four treatment groups (n/group=6). Data are shown as mean±SEM.

Dongdong Li, et al. J Neurochem. ;118(2):237-247.
2.
Figure 3

Figure 3. From: Casein kinase 1 enables nucleus accumbens amphetamine induced locomotion by regulating AMPA receptor phosphorylation.

PF blocks NAcc AMPH induced inhibition of PP1 activity and phosphorylation of GluR1 at S845. (A) PP1 activity (**, P<0.01, vs Saline; ††, P<0.01, vs AMPH; Scheffé), and (C) pGluR1(S845) (***, P<0.001, vs Saline; †††, P<0.001, vs AMPH; Scheffé), (D) GluR1, and (F) pGluR1(S831) protein levels in the NAcc of rats in the four treatment groups (n/group=6). Data are shown as mean±SEM. Representative immunoblots of (B) pGluR1(S845) and (E) pGluR1(S831) and GluR1 in the NAcc of rats in the four treatment groups.

Dongdong Li, et al. J Neurochem. ;118(2):237-247.
3.
Figure 1

Figure 1. From: Casein kinase 1 enables nucleus accumbens amphetamine induced locomotion by regulating AMPA receptor phosphorylation.

The Csnk1δ/ε inhibitor PF-670462 (PF) dose-dependently blocks NAcc amphetamine (AMPH) induced locomotion. (A) Locomotor activity counts before and after NAcc AMPH (arrows) in three experiments each testing the indicated dose of PF (n/group=6-8). Insets show 60-minute total locomotor counts following AMPH (*, P<0.05, ***, P<0.001, vs Saline; ††, P<0.01, vs AMPH; Scheffé). (B) 60-minute total locomotor counts following NAcc saline or PF alone (n/group=6-7). (C) Injection cannula placements in the NAcc core. The numbers to the right indicate millimeters from bregma (Paxinos and Watson, 1997). Data are shown as mean±SEM.

Dongdong Li, et al. J Neurochem. ;118(2):237-247.
4.
Figure 4

Figure 4. From: Casein kinase 1 enables nucleus accumbens amphetamine induced locomotion by regulating AMPA receptor phosphorylation.

Viral mediated expression of GluR1(S845A) in cultured medium spiny neurons blocks SKF81297-evoked phosphorylation of GluR1 and glutamate-evoked currents. (A) Photomicrographs of neurons that were (left) not infected, (middle) infected with Lenti-GFP, or (right) infected with Lenti-GluR1(S845A). Scale bar = 20μm. (B) Representative immunoblots of pGluR1(S845) and GluR1, (C) pGluR1(S845) (***, P<0.001, vs remaining groups; Scheffé), and (D) GluR1 protein levels in the three groups of neurons either incubated or not incubated with SKF81297. n/group=4-7. (E) Representative current traces obtained in the three groups of neurons before and after focal application of glutamate (arrowheads). (F) Mean peak currents obtained following glutamate in the three groups of neurons (**, P<0.01, vs Control non-infected cells; Scheffé). n/group=5-12. Data are shown as mean±SEM.

Dongdong Li, et al. J Neurochem. ;118(2):237-247.
5.
Figure 5

Figure 5. From: Casein kinase 1 enables nucleus accumbens amphetamine induced locomotion by regulating AMPA receptor phosphorylation.

Viral mediated expression of GluR1(S845A) in the NAcc blocks AMPH-induced phosphorylation of GluR1 and AMPH-induced locomotion. (A) Representative immunoblots of GluR1(S845), GluR1, and Cop GFP, (B) pGluR1(S845) (***, P<0.001, vs remaining groups; Scheffé), and (C) GluR1 protein levels in rats not infected (Control), infected with Lenti-GFP, or infected with Lenti-GluR1(S845A) either challenged or not challenged with NAcc AMPH. n/group=5-6. (D) Photomicrographs of the NAcc obtained 3 weeks following infection with Lenti-GFP or Lenti-GluR1(S845A) illustrating GFP positive neurons in close proximity to the injection cannula tips (arrows). Scale bar = 50μm. (E) Locomotor activity counts obtained before and after (arrow) NAcc AMPH in the two groups (*, P<0.05, ***, P<0.001, vs Lenti-GFP; Scheffé). n/group=5-6. Data are shown as mean±SEM.

Dongdong Li, et al. J Neurochem. ;118(2):237-247.

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