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1.
Figure 3

Figure 3. Fluorescence microscopy of the Imp3-GFP and Imp3Q-GFP fusions.. From: A Viable Hypomorphic Allele of the Essential IMP3 Gene Reveals Novel Protein Functions in Saccharomyces cerevisiae.

Localization of both fusions was observed in the wild-type strain grown on plasmid-selective minimum medium at 30°C.

Bruno Cosnier, et al. PLoS One. 2011;6(4):e19500.
2.
Figure 1

Figure 1. Imp3Qp expression and phenotypes of the IMPQ strain.. From: A Viable Hypomorphic Allele of the Essential IMP3 Gene Reveals Novel Protein Functions in Saccharomyces cerevisiae.

(A) Western blot analysis of the Imp3Q and Imp3 protein levels. HA-tagged proteins were expressed in the wild-type FS1 strain. (B) IMPQ growth phenotypes: serial dilutions of FS1 and IMPQ cultures grown on YPD at 20°C and 30°C for 3 days.

Bruno Cosnier, et al. PLoS One. 2011;6(4):e19500.
3.
Figure 4

Figure 4. Translational accuracy in the IMPQ strain.. From: A Viable Hypomorphic Allele of the Essential IMP3 Gene Reveals Novel Protein Functions in Saccharomyces cerevisiae.

The median recoding efficiency of at least five independent experiments is indicated for each target sequence assayed (TAG, TGA and TAA for readthrough, IBV (Avian infectious bronchitis virus) for −1 frameshifting, EST3 and OAZ for +1 frameshifting). Results significantly different between the IMPQ and FS1 strains are marked by an asterisk (p-value<0.0001).

Bruno Cosnier, et al. PLoS One. 2011;6(4):e19500.
4.
Figure 5

Figure 5. Drug sensitivity of the IMPQ strain.. From: A Viable Hypomorphic Allele of the Essential IMP3 Gene Reveals Novel Protein Functions in Saccharomyces cerevisiae.

Growth of the FS1 and IMPQ strains is shown on YPD plates containing various concentrations of (A) aminoglycosides (Paromomycin 500 µg/mL, G418 25 µg/mL), or (B) DNA damaging agents (Bleomycin 1 µg/mL, Phleomycin 1 µg/mL, Camptothecin 20 µg/mL, Hydroxyurea (HU) 0.1M, Methyl methanesulfonate (MMS) 0.5%, H2O2 1 µg/mL). Culture of the IMPQ strain complemented by pCEN-IMP3 was also assayed on phleomycin (1 µg/mL).

Bruno Cosnier, et al. PLoS One. 2011;6(4):e19500.
5.
Figure 6

Figure 6. Analysis of telomere length in wild-type and IMPQ strains.. From: A Viable Hypomorphic Allele of the Essential IMP3 Gene Reveals Novel Protein Functions in Saccharomyces cerevisiae.

(A) Genomic DNA from two independent clones digested with XhoI and probed with telomere-specific probe, detecting TG1–3 repeats. Telomere ends are bracketed, the 1 kb DNA ladder (NEB) was migrated in the first and last lines, and internal migration control is the sharp band of 2.5 kb. FS1 and IMPQ strains were grown in YPD medium overnight at 30°C, FS1 and IMPQ strains harbouring pCEN and pCEN-IMP3 plasmids were grown in CSM-URA medium in the same conditions. (B) Difference in telomere length is indicated between various strains.

Bruno Cosnier, et al. PLoS One. 2011;6(4):e19500.
6.
Figure 2

Figure 2. Polysome profile and pre-rRNA processing defects of the IMPQ strain.. From: A Viable Hypomorphic Allele of the Essential IMP3 Gene Reveals Novel Protein Functions in Saccharomyces cerevisiae.

(A) Polysome profile of the FS1 and IMPQ strains. Equivalent amounts of polysome extracts were analyzed by sucrose gradients in each case. The positions of 40S and 60S subunits are indicated as well as the monosomes (80S) and polysome peaks (B) Northern blot analyses of pre-rRNA and mature rRNA in the FS1 (lanes 1, 3, 5, 7, 9) and IMPQ (lanes 2, 4, 6, 8, 10) strains. The 35S rRNA precursor is schematically represented on the top of the figure, with the position of all oligonucleotides used as probes: (18)+(25) lanes 1, 2; (a) lanes 3, 4; (b) lanes 5, 6; (c) lanes 7, 8; (d) lanes 9, 10. The 23S, 20S and 27SA2 species are represented by bars. Non-specific signals were obtained for 18S and 25S RNA in (a), (b), (c) and (d) blots.

Bruno Cosnier, et al. PLoS One. 2011;6(4):e19500.

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