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Results: 5

1.
Figure 4

Figure 4. From: Berberine acutely activates the glucose transport activity of GLUT1.

Kinetics of 2DG uptake in control and berberine-activated cells. L929 fibroblast cells were incubated at 37 °C for 30 minutes in DMEM media (5.5 mM glucose) supplemented with either 0 or 80 μM berberine. Ten-minute 2DG uptakes were then measured at varying concentrations of 2DG (0.1, 0.5, 1, 5, 10, or 20 mM). Data are means ± S.E. of a representative experiment.

Alexandra Cok, et al. Biochimie. ;93(7):1187-1192.
2.
Figure 1

Figure 1. From: Berberine acutely activates the glucose transport activity of GLUT1.

Effects of berberine on 2DG uptake. L929 fibroblast cells were incubated at 37 °C for 30 minutes in DMEM media (5.5 mM glucose) supplemented with 0-100 μM berberine as indicated. Ten-minute 2DG uptakes were then measured as described in Materials and Methods. Data are means ± S.E. of normalized values from multiple experiments (samples size varied from 3-20). aSignificantly different than 0 μM berberine and bsignificantly different than 100 μM berberine at P<0.01.

Alexandra Cok, et al. Biochimie. ;93(7):1187-1192.
3.
Figure 5

Figure 5. From: Berberine acutely activates the glucose transport activity of GLUT1.

Effect of Compound C on berberine activated 2DG uptake. L929 fibroblast cells were pretreated for 45 minutes at 37 °C in DMEM media (5.5 mM glucose) supplemented with 0, 10, 20, or 50 μM compound C, then incubated for 30 min in 5.5 mM glucose DMEM media still supplemented with compound C and either 0 or 50 μM berberine. Ten-minute 2DG uptakes were then measured as described in Materials and Methods. Data are means ± S.E. of a representative experiment.

Alexandra Cok, et al. Biochimie. ;93(7):1187-1192.
4.
Figure 3

Figure 3. From: Berberine acutely activates the glucose transport activity of GLUT1.

Effect of recovery time on berberine-activated 2DG uptake. L929 fibroblast cells were incubated at 37 °C for 30 min in 5.5 mM glucose DMEM media supplemented with 40 μM berberine. Samples were washed and resuspended in media without berberine for 0-60 min. Ten-minute 2DG uptakes were then measured as described in Materials and Methods. Data are means ± S.E. of a representative experiment. 2DG uptake rates at all recovery times are statistically different from the control (0 min) at P<0.05.

Alexandra Cok, et al. Biochimie. ;93(7):1187-1192.
5.
Figure 2

Figure 2. From: Berberine acutely activates the glucose transport activity of GLUT1.

Effect of time of berberine exposure on 2DG uptake. L929 fibroblast cells were incubated at 37 °C for 30 minutes in DMEM media (5.5 mM glucose) supplemented with 40 μM berberine for 0, 5, 10, 30, 45, and 60 min. Ten-minute 2DG uptakes were then measured as described in Materials and Methods. Data are means ± S.E. of a representative experiment. aSignificantly different than 0 minute exposure to berberine and bsignificantly different than one hour at P<0.01.

Alexandra Cok, et al. Biochimie. ;93(7):1187-1192.

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