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Results: 3

1.
Figure 1

Figure 1. From: Inhibition of full length Hepatitis C Virus particles of 1a genotype through small interference RNA.

Toxicity of siRNAs through MTT cell proliferation assay. Huh-7 cells were plated at the density of 2 × 104 in 96 well plates. After 24 h cells were treated with different concentrations of siRNAs and control consisted of solvent in which siRNAs dissolved. After 24 h incubation period add MTT solution to all wells and incubated for 3-4 h at 37°C. Viable cells convert MTT to purple formazan crystal. Added DMSO to dissolve the formazan crystals and read absorbance at 570 nm and 620 nm.

Muhammad Ansar, et al. Virol J. 2011;8:203-203.
2.
Figure 2

Figure 2. From: Inhibition of full length Hepatitis C Virus particles of 1a genotype through small interference RNA.

Silencing effect of core specific siRNAs against full length viral particles of HCV 1a genotype. Huh-7 cells were infected with high titer sera sample from HCV-1a patients (S1a) to establish in vitro cell culture model of HCV-1a, cells were maintained overnight at 37°C in 5% CO2 for three days. Cells were harvested after siRNA treatment 48 hrs post transfection and intracellular HCV RNA levels were quantified by Real Time PCR. Data is expressed as percentage of HCV survival in cells. S1a means control consisted of HCV serum of 1a genotype) and SC means scrambled siRNA. Error bars indicate, mean S.D p < 0.05 verses S1a.

Muhammad Ansar, et al. Virol J. 2011;8:203-203.
3.
Figure 3

Figure 3. From: Inhibition of full length Hepatitis C Virus particles of 1a genotype through small interference RNA.

Silencing effect of HCV E1 and E2 specific siRNAs against full length viral particles of HCV 1a genotype. Huh-7 cells were infected with high titer sera sample from HCV-1a patients (S1a) to establish in vitro cell culture model of HCV-1a, cells were maintained overnight at 37°C in 5% CO2 for three days. Cells were harvested after siRNA treatment 48 hrs post transfection and intracellular HCV RNA levels were quantified by Real Time PCR. Data is expressed as percentage of HCV survival in cells. S1a means control consisted of HCV serum of 1a genotype) and SC means scrambled siRNA. Error bars indicate, mean S.D p < 0.05 verses S1a.

Muhammad Ansar, et al. Virol J. 2011;8:203-203.

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