Results: 4

1.
Figure 2

Figure 2. From: Quantitative Assessment Shows Loss of Antigenic Epitopes as a Function of Pre-analytic Variables.

Comparison of biomarker staining images between biopsies vs. tumor resections on whole tissue section slides. Representative immunofluorescence staining of p-AKT (Red) in CNB (A) and tumor resection (B), AKT (red) in CNB (C) and tumor resection (D), ER (red) in CNB (E) and tumor resection (F), GAPDH (red) in biopsy (G) and tumor resection (H) were illustrated. Each corresponding Cytokeratin staining is shown as inset (Green). Photographs are shown at magnification of 20×.

Yalai Bai, et al. Lab Invest. ;91(8):1253-1261.
2.
Figure 1

Figure 1. From: Quantitative Assessment Shows Loss of Antigenic Epitopes as a Function of Pre-analytic Variables.

Differences in biomarker expression in core needle biopsies vs. tumor resections. Twenty core needle biopsies and matched tumor resections were arrayed in TMA with 2-fold redundancy. 1.5-mm core from each tumor block was arrayed in a recipient block. The TMA was immunohistochemically stained with ER, p53, Ki67, pERK, pAKT and pTyr and the results were quantified using AQUA. Scores represent the average of two cores. Specimens that showed decreased staining in the resection relative to biopsy are shown in green; those with higher resection levels are shown in red.

Yalai Bai, et al. Lab Invest. ;91(8):1253-1261.
3.
Figure 4

Figure 4. From: Quantitative Assessment Shows Loss of Antigenic Epitopes as a Function of Pre-analytic Variables.

Epitope-specific protein degradation in tumor resections. AQUA scores of each coded biopsy (core needle biopsy, open bars) and tumor resection (tumor resection, filled bars) in staining of ER (A), Ki67 (B), GAPDH (C), Pan-cytokeratin (D) were assessed. Raw Pan-cytokeratin scores were scaled to a 100-point scale. The number on the top of each bar indicates number of field of view (FOV)) included in the observation. Each AQUA data represents the mean ± 95% CI. n represents the number of FOVs. Comparisons between core needle biopsiess and tumor resections were performed via the Wilcoxon Signed Rank test.

Yalai Bai, et al. Lab Invest. ;91(8):1253-1261.
4.
Figure 3

Figure 3. From: Quantitative Assessment Shows Loss of Antigenic Epitopes as a Function of Pre-analytic Variables.

Expression of phospho-proteins including pAKT, pERK and pS6K1 were decreased in tumor resections compared to those in biopsies. AQUA scores of each coded biopsy (core needle biopsies, open bars) and tumor resection (tumor resection, filled bars) in staining of pAKT (A), AKT (B), pERK (C), ERK (D), pS6K1 (E) and S6K1 (F) were assessed. The number on the top of each bar indicates number of field of view (FOV) included in the observation. Each AQUA data represents the mean ± 95% CI. N represents the number of FOVs. Comparisons between core needle biopsies and tumor resections were performed via the Wilcoxon Signed Rank test.

Yalai Bai, et al. Lab Invest. ;91(8):1253-1261.

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