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Results: 9

1.
FIGURE 6

FIGURE 6. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

Histology of colon cross sections. A. Lum+/+ and B. Lum−/− saline and TNBS treated mice. C. Lum+/+ and D. Lum−/− TNBS treated colons under low magnification show inflammation of the submucosa (SM) and some inflammatory cell infiltrates in the lamina propria (LP). Necrosis and tissue damage is shown under high magnification in E. Lum+/+ and F. Lum−/− TNBS treated mice.

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.
2.
FIGURE 5

FIGURE 5. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

TNBS colitis in the Rag1−/− mice. A. The mice were given an intra-rectal treatment of TNBS or saline and weighed daily. Weight loss in the TNBS treated wild type and the Rag1−/− was similar. B. Macroscopic appearance of the colon. The colon appeared equally inflamed in the wild type and the Rag1−/− mice. C. Histology. Colonic sections of the TNBS treated Rag1−/− mice showed signs of inflammation that were comparable to those seen in the wild type. The submucosa was inflamed and edematous and inflammatory cells were visible in the submucosa and the lamina propria.

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.
3.
FIGURE 1

FIGURE 1. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

TNBS Colitis model. The mice were pre-sensitized by epicutaneous application of 1% TNBS in 80% ethanol. Seven days later 5 µl/g body weight of 2.5% TNBS in 50% ethanol was administered intra-rectally under anesthesia. The mice were euthanized 48 hours later for tissue harvest, or followed up to 8 days after TNBS treatment for daily body weight measurements.

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.
4.
FIGURE 3

FIGURE 3. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

Macroscopic appearance of the colon in TNBS-treated Lum+/+ and Lum−/− C57BL/6J mice. The colons were harvested 48 hours after intra-rectal TNBS administration and photographed. Saline treated colons (top two panels) showed no inflammation. TNBS treated colons with mild inflammation (middle two panels) appeared bloody in areas (arrows), while colons showing significant inflammation (bottom panels) were macroscopically thickened and necrotic (arrow heads). The highly inflamed colons were seen more frequently in the Lum+/+ TNBS-treated mice. This is one of three representative experiments.

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.
5.
FIGURE 8

FIGURE 8. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

Cytokine levels in colonic protein extracts. A. CXCL1/KC was increased significantly (p=0.01) in colonic extracts of Lum+/+ TNBS-treated mice compared to saline treated controls. The Lum−/− mice showed little increase in KC after TNBS treatment. B. TNFα levels were increased significantly (p=0.012) in the Lum+/+ TNBS mice, while there was no significant increase in TNF-α in Lum−/− TNBS compared to saline-treated controls. C. IL-4 levels showed no significant increase after TNBS treatment in the Lum+/+ or the Lum−/− mice. * A p value ≤ 0.05 was considered statistically significant based on Mann-Whitney test. The result shown is one of two similar experiments.

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.
6.
FIGURE 7

FIGURE 7. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

Reduced influx of neutrophils in Lum−/− mice compared to Lum+/+ TNBS treated colons but increased overall tissue damage. A. Tiled bar graph showing percent of animals with 0–4 inflammation scores (Methods). In this experimental set there were no mice that displayed scores of 3 or 4. Overall, the Lum−/− TNBS challenged group showed more animals with an inflammation score of 2. B. Measurements of MPO by ELISA to assess neutrophil infiltration after TNBS challenge. TNBS treated Lum−/− showed no significant increases in MPO, whereas Lum+/+ TNBS treated colons showed significant increases (*p=0.048, Mann-Whitney test).

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.
7.
FIGURE 4

FIGURE 4. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

Increased thickening of TNBS treated colons. A. Cross-sectional areas of colons, taken as a measure of colon thickening and inflammation for Lum+/+ and Lum−/− C57BL/6J mice treated with TNBS. Colon cross-sectional areas were increased significantly in Lum−/− TNBS treated mice compared to saline controls. * p=0.02 (Mann-Whitney test). The data shown is one of two representative experiments. B. Increased colon to body weight ratio in Lum+/+ and Lum−/− CD1 mice treated with TNBS. The increase in colon to body weight ratio was significantly increased in TNBS challenged Lum+/+ (p= 0.005) and Lum−/− (p=0.0002) mice compared to their saline treated controls. * A p value ≤ 0.05 was considered statistically significant based on Mann-Whitney test. Representative data from two similar experiments is shown here.

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.
8.
FIGURE 9

FIGURE 9. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

Poor NF-κB response in the absence of lumican. A. Total NF-κB level was assessed in colonic extracts by ELISA measurements of the p65 subunit. The increase in total NF-κB in TNBS treated mice of both genotypes was significant (p ≤ 0.05) compared to the saline controls. However, the increase was much lower in the Lum−/− (1.7X) compared to that seen in the Lum+/+ (2.5X) mice. B. Delayed nuclear localization of NF-κB in peritoneal macrophages of Lum−/− mice compared to Lum+/+ after exposure to 10 ng/ml LPS. Nuclear extracts were tested by EMSA (Methods). Lum−/− peritoneal macrophages show maximal NF-κB binding after 20 min LPS exposure, while this was achieved by 10 min in Lum+/+ macrophages.

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.
9.
FIGURE 2

FIGURE 2. From: Extracellular matrix protein lumican regulates inflammation in a mouse model of colitis.

Response to intra-rectal TNBS treatment. A. Lum+/+ and Lum−/− in the C57BL/6J mice were challenged with 2.5%TNBS in 50% ethanol (ETOH) or ETOH alone. Change in body weight was expressed as a percent weight change relative to their weight on day 0. The Lum−/− TNBS challenged mice failed to recover the lost body weight, whereas the ETOH challenged Lum−/− and the wild type TNBS and ETOH treated mice recovered by day 6. B. TNBS colitis using saline treatment as a control. The saline treated mice did not lose weight while the TNBS treated mice lost weight. C. Survival presented as a Kaplan Meier survival plot. Lum+/+ and Lum−/− C57BL/6J mice challenged with or without TNBS as in Figure 1, and observed for survival for 8 days. There was no significant difference in survival between the two genotypes. The data shown here is one of two representative experiments.

Kristin Lohr, et al. Inflamm Bowel Dis. ;18(1):143-151.

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