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Results: 5

1.
Figure 1

Figure 1. From: Application of Fiber Optic ATR-FTIR Methods for In Situ Characterization of Protein Delivery Systems in Real Time.

Schematic of the in situ ATR-FTIR sample vessels a) hydrogel lies in the bottom of a vial with the acidic buffer and probe above b) three-neck round bottom flask allows access to the nanoparticle suspension for the probe, pH electrode, and acid.

Cathryn L. McFearin, et al. Anal Chem. ;83(10):3943-3949.
2.
Figure 5

Figure 5. From: Application of Fiber Optic ATR-FTIR Methods for In Situ Characterization of Protein Delivery Systems in Real Time.

Top: Peak absorption versus pH for the ester and amide I vibrations Bottom: Random copolymer IR spectra at different solution pH values during particle degradation. Gray arrows indicate direction of amide bond absorbance trend with lowered pH.

Cathryn L. McFearin, et al. Anal Chem. ;83(10):3943-3949.
3.
Figure 4

Figure 4. From: Application of Fiber Optic ATR-FTIR Methods for In Situ Characterization of Protein Delivery Systems in Real Time.

a) IR spectra of empty particles formulated from the random copolymer at different pH values. Mechanism showing exposure to mildly acidic conditions facilitates Step 1: protonation of amines allowing Step 2: hydration followed by fragmentation of polymer. b) Ester peak heights as a function of pH. Cartoon of random copolymer particle degradation.

Cathryn L. McFearin, et al. Anal Chem. ;83(10):3943-3949.
4.
Figure 3

Figure 3. From: Application of Fiber Optic ATR-FTIR Methods for In Situ Characterization of Protein Delivery Systems in Real Time.

a) IR spectra and major vibrational assignments of PEGA hydrogels formulated with the acid degradable crosslinker (black trace) and a non-acid degradable crosslinker bisacrylamide (red trace) with no encapsulated protein and the chemical structure of PEGA b) Peak height to a single baseline IR absorbance of the amide I C=O stretch of released HSA at pH 5 over time from hydrogels formulated with the acid degradable crosslinker (black squares) and a non-acid degradable crosslinker bisacrylamide (red circles). Markers are data points and the solid lines are fits to the data.

Cathryn L. McFearin, et al. Anal Chem. ;83(10):3943-3949.
5.
Figure 2

Figure 2. From: Application of Fiber Optic ATR-FTIR Methods for In Situ Characterization of Protein Delivery Systems in Real Time.

a) IR spectra and major vibrational assignments of polyacrylamide hydrogels formulated with the acid degradable crosslinker (black trace) and a non-acid degradable crosslinker bisacrylamide (red trace) with no encapsulated protein and the chemical structure of the acid degradable crosslinker b) Peak height to a single baseline IR absorbance of the amide I C=O stretch of released HSA at pH 5 over time from hydrogels formulated with the acid degradable crosslinker (black squares) and a non-acid degradable crosslinker bisacrylamide (red circles). Markers are data points and the solid lines are fits to the data. Inset: IR spectrum after hydrogel degradation showing the polyacrylamide vibrational bands in addition to the released protein.

Cathryn L. McFearin, et al. Anal Chem. ;83(10):3943-3949.

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