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1.
Scheme 1.

Scheme 1. From: Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing ?-cell function.

(A) Conjugation schemes to synthesize thiolated EphA5-Fc and ehprinA5-Fc. (B) Thiol-acrylate photopolymerization used to fabricate cell-laden biomimetic hydrogels.

Chien-Chi Lin, et al. Proc Natl Acad Sci U S A. 2011 April 19;108(16):6380-6385.
2.
Fig. 5.

Fig. 5. From: Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing ?-cell function.

Representative confocal Z-stack (300 μm) image of encapsulated MIN6 cells stained with live/dead viability staining kit 21 days after encapsulation. Thiolated EphA5/ephrinA5–Fc: 200 nM (1∶1). Initial MIN6 cell-packing density: 6.7 × 106 cells/mL. Live cells were stained green while dead cells were stained red (scale: 200 μM).

Chien-Chi Lin, et al. Proc Natl Acad Sci U S A. 2011 April 19;108(16):6380-6385.
3.
Fig. 4.

Fig. 4. From: Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing ?-cell function.

(A) Intracellular ATP of encapsulated MIN6 cells at the indicated time after encapsulation as a measure of cell viability (Mean ± SEM, n = 3). (B) Representative confocal Z-stack (300 μm) images of encapsulated MIN6 cells stained with live/dead viability staining kit in EphA5/ephrinA5 dual immobilized hydrogels with indicated concentrations. Live cells stained green while dead cells stained red (scale: 200 μM).

Chien-Chi Lin, et al. Proc Natl Acad Sci U S A. 2011 April 19;108(16):6380-6385.
4.
Fig. 6.

Fig. 6. From: Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing ?-cell function.

(A) Initial viability of MIN6 cells encapsulated (2 × 106 cells/mL) in PEG hydrogels functionalized with different concentrations of CGRGDS and either 200 nM Fc (white bars) or 100 nM EphA5–Fc and 100 nM ephrin–A5–Fc (gray bars). Asterisks indicate statistical significance between assigned groups (* p < 0.05) (Mean ± SEM, n = 3). (B) Initial viability of dissociated islet cells encapsulated in PEG hydrogels functionalized with 100 μM CGRGDS and 200 nM thiolated IgG–Fc or thiolated EphA5–Fc and ephrin–A5–Fc (1∶1). Asterisks indicate statistically significance between assigned groups (* p < 0.05) (Mean ± SEM, n = 3).

Chien-Chi Lin, et al. Proc Natl Acad Sci U S A. 2011 April 19;108(16):6380-6385.
5.
Fig. 3.

Fig. 3. From: Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing ?-cell function.

(A) Effects of EphA5–Fc and ephrinA5–Fc immobilization on viability of MIN6 cells in PEG hydrogels. MIN6 cells were encapsulated at 6.7 × 106/mL. (A) Intracellular ATP of encapsulated MIN6 cells was quantified 1 hr after encapsulation. Hydrogels immobilized with Fc protein were used as controls (Mean ± SEM, n = 3 or 4). (B) Initial viability of MIN6 cells encapsulated (6.7 × 106 cells/mL) in PEG hydrogels functionalized with 200 nM Fc (white bar), 200 nM total of thiolated (light gray bar), or nonthiolated EphA5–Fc and ephrin–A5–Fc (dark gray bar). Asterisks indicate statistical significance between assigned groups (* p < 0.05) (Mean ± SEM, n = 3).

Chien-Chi Lin, et al. Proc Natl Acad Sci U S A. 2011 April 19;108(16):6380-6385.
6.
Fig. 1.

Fig. 1. From: Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing ?-cell function.

Effects of cell-packing density on the survival of MIN6 cells in PEG hydrogels. MIN6 cells were dispersed into single cells and encapsulated at different cell densities as indicated. Cell-laden hydrogels were maintained in RPMI-1640 medium containing 1% FBS. (A) Intracellular ATP of encapsulated MIN6 cells was measured at the indicated time after encapsulation and was used to represent cell metabolic activity and viability (Mean ± SEM, n = 3). (B) Representative confocal Z-stack (300 μm) images of encapsulated MIN6 cells stained with a live/dead viability staining kit at day-0 and day-10. Live cells were stained green while dead cells were stained red (scale: 200 μM).

Chien-Chi Lin, et al. Proc Natl Acad Sci U S A. 2011 April 19;108(16):6380-6385.
7.
Fig. 2.

Fig. 2. From: Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing ?-cell function.

Effects of MIN6 cell-packing density in PEG hydrogels on glucose responsive insulin secretion. MIN6 cells were dispersed into single cells and encapsulated at different cell densities as indicated. Cell-laden hydrogels were maintained in RPMI-1640 medium containing 10% FBS for 10 days before insulin secretion was tested in Krebs–Ringer buffer solution (KRB). The amount of insulin secreted from samples incubated in 25 mM glucose KRB was normalized to the amount of insulin secreted from the respective samples incubated in 2 mM glucose KRB and expressed as an insulin secretion index (Mean ± SEM, n = 4). All values of the insulin secretion index were significantly different from 1 (p < 0.05), with & indicating lower and * indicating higher than 1.

Chien-Chi Lin, et al. Proc Natl Acad Sci U S A. 2011 April 19;108(16):6380-6385.

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