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Results: 4

1.
Figure 3

Figure 3. From: Recombination Phenotypes of Escherichia coli greA Mutants.

Transduction. Strains were infected with an amount of a P1 lysate grown on a metBΔtet strain sufficient to generate 50-100 transductants in the wild type. Ratios of mutant transductants to wild type transductants were calculated. Means and standard errors of 3-8 crosses are shown. A. RecBCD+ strains. B. Strains in which recBCD is replaced by red.

Anthony R Poteete. BMC Mol Biol. 2011;12:12-12.
2.
Figure 4

Figure 4. From: Recombination Phenotypes of Escherichia coli greA Mutants.

Sequence of the 587-bp dsDNA used in the tests of Red-mediated chromosomal gene replacement. The first and last 50 bases are the homology flanks. Bases in bold type are, in order, the start codon of bla (reverse complement), the -35 and -10 hexamers of P32, and lacZ ribosome binding site.

Anthony R Poteete. BMC Mol Biol. 2011;12:12-12.
3.
Figure 1

Figure 1. From: Recombination Phenotypes of Escherichia coli greA Mutants.

Marker rescue. A. λ cI857 R-am5, bearing an amber mutation in the endolysin gene R, can form a plaque on the non-suppressing host only if it recombines with the plasmid or reverts. B. Efficiencies of mutants relative to wild type. Titers of the phage on cells bearing the homology-containing plasmid, minus titers on cells bearing the vector (revertants) are determined, and divided by the wild type value. Means and standard errors of four experiments are shown. All strains are recA.

Anthony R Poteete. BMC Mol Biol. 2011;12:12-12.
4.
Figure 2

Figure 2. From: Recombination Phenotypes of Escherichia coli greA Mutants.

Red-mediated chromosomal gene replacement. A. Strains bearing an alteration of the lac locus in which lacI through the N-terminal coding region of lacZ is replaced by the C-terminal two-thirds of the bla gene and the aph gene of Tn903 can recombine with a linear dsDNA generated by PCR and introduced by electroporation, creating an ampicillin-resistant, Lac+ recombinant. B. Efficiencies of mutant strains relative to wild type. Means and standard errors of three or four experiments are shown.

Anthony R Poteete. BMC Mol Biol. 2011;12:12-12.

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