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Results: 6

1.
Fig. 6

Fig. 6. From: Inhibitors of histone demethylation and histone deacetylation cooperate in regulating gene expression and inhibiting growth in human breast cancer cells.

Validation of gene expression induced by combination of SAHA and pargyline. mRNA was measured by quantitative real time PCR for indicated gene expression

Yi Huang, et al. Breast Cancer Res Treat. 2012 February;131(3):777-789.
2.
Fig. 1

Fig. 1. From: Inhibitors of histone demethylation and histone deacetylation cooperate in regulating gene expression and inhibiting growth in human breast cancer cells.

Inhibition of LSD1 activity by HDAC inhibitors. a MDA-MB-231 and MDA-MB-468 cells were exposed to indicated HDAC inhibitors for 24 h. b Cells were treated with splitomicin or nicotinamide for 24 h. 30 µg of nuclear protein/lane were analyzed by immunoblots for expression of H3K4me2 or AcH3K9. Nuclear lysate of MDA-MB-231 cells treated with 5 µM SAHA for 24 h was used as positive control for LSD1 inhibition. H3 was used as a loading control

Yi Huang, et al. Breast Cancer Res Treat. 2012 February;131(3):777-789.
3.
Fig. 5

Fig. 5. From: Inhibitors of histone demethylation and histone deacetylation cooperate in regulating gene expression and inhibiting growth in human breast cancer cells.

Genome-wide microarray analysis. a MDA-MB-231 cells were treated with 5 µM SAHA, 2.5 mM pargyline alone or in combination for 24 h. Microarray analysis was performed. Diagrams of up-regulated or down-regulated genes by SAHA, pargyline or combination were shown. b Expression profiles of genes that displayed ≥1.5 fold induction after combined treatment. c Expression profile of genes that displayed ≥1.5 fold reduction after combined treatment. Shown is the mean ±SD for three replicates

Yi Huang, et al. Breast Cancer Res Treat. 2012 February;131(3):777-789.
4.
Fig. 2

Fig. 2. From: Inhibitors of histone demethylation and histone deacetylation cooperate in regulating gene expression and inhibiting growth in human breast cancer cells.

Effects of pargyline on histone marks. a MDA-MB-231 and MDA-MB-468 cells were treated with increasing concentrations of pargyline for 48 h. MTT assays were performed. * P < 0.05, *** P < 0.001, (pargyline vs. control, Student’s t-test). b MDA-MB-231 cells were treated with indicated concentrations of pargyline for 24 h and analyzed by immunoblots for expression of H3K4me2, H3k4me1, and AcH3. c MDA-MB-231 cells were exposed to 5 µM pargyline or 5 mM SAHA for 24 h and analyzed for expression of indicated proteins by immunoblots. H3 was used as a loading control

Yi Huang, et al. Breast Cancer Res Treat. 2012 February;131(3):777-789.
5.
Fig. 4

Fig. 4. From: Inhibitors of histone demethylation and histone deacetylation cooperate in regulating gene expression and inhibiting growth in human breast cancer cells.

LSD1 and HDAC inhibitors exhibit synergistic growth inhibition. a MDA-MB-231 cells were exposed to 1 mM pargyline or 1 µM SAHA alone or in combination for 24 h and were analyzed for expression of H3K4me2 and AcH3K9. H3 was used as a loading control. b Cells were simultaneously treated with pargyline or HDAC inhibitors for 48 h. The combination index (CI) values shown represent the mean ±SD for three independent experiments

Yi Huang, et al. Breast Cancer Res Treat. 2012 February;131(3):777-789.
6.
Fig. 3

Fig. 3. From: Inhibitors of histone demethylation and histone deacetylation cooperate in regulating gene expression and inhibiting growth in human breast cancer cells.

Knockdown of LSD1 by siRNA leads to increase of histone acetylation. a MDAMB-231 cells were transfected with mock, scrambled, or LSD1-targeted siRNA oligonucleotides for 48 h and subjected to immunoblotting with indicated antibodies. b After LSD1 siRNA transfection, 30 µg of nuclear protein/lane were analyzed by immunoblots for expression of H3K4me2 and AcH3K9. c After MDA-MB-231 cells were transfected with mock, scrambled, or LSD2 siRNA. mRNA was measured by quantitative real time PCR analysis for LSD2 gene expression. Results represent the mean of three independent experiments, each performed in triplicate ±SD. *** P < 0.001, (siRNA vs. mock or scramble, Student’s t-test). d After LSD2 siRNA transfection, nuclear proteins were analyzed for expression of H3K4me2 and AcH3K9. H3 was used as a loading control

Yi Huang, et al. Breast Cancer Res Treat. 2012 February;131(3):777-789.

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