Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 8

1.
Figure 4

Figure 4. From: Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells.

Brain lacZ staining in TRPV1Cre/R26R-lacZ mice. (A) Schematic showing the Trpv1 locus of TRPV1Cre mice. (B-C) lacZ staining in DRG (B) and SC (C) of TRPV1Cre/R26R-lacZ mice. We observed lacZ+ cells in the IF (D), entorhinal cortex (E), rostral linear raphe (RLi) (F), SuM (G), periaqueductal gray (PAG) (H), Cajal-Retzius cells of the hippocampus (HI) (I), DMH (J), and AOB (K). Insets are magnified images of dashed regions. Scale bar = 100 μm in B and 200 μm in C-K.

Daniel J. Cavanaugh, et al. J Neurosci. ;31(13):5067-5077.
2.
Figure 2

Figure 2. From: Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells.

nlacZ staining in coronal sections of TRPV1PLAP-nlacZ mice. We observed sparse nlacZ expression in the IF (A), entorhinal cortex (B), rostral linear raphe (RLi), (C), supramammillary nucleus (SuM) (D), periaqueductal gray (PAG) (E), Cajal-Retzius cells of the hippocampus (HI) (F), dorsomedial hypothalamus (DMH) (G), and accessory olfactory bulb (AOB) (H). Insets in A, C, D, E and G are magnified images of dashed regions. Insets in F and H show sections processed for nlacZ (magenta) and stained for reelin immunoreactivity (green). (I) Outlines of coronal brains illustrating the nlacZ+ areas of the rostral midbrain and caudal hypothalamus. Scale bar = 200 μm.

Daniel J. Cavanaugh, et al. J Neurosci. ;31(13):5067-5077.
3.
Figure 8

Figure 8. From: Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells.

Calcium imaging of TRPV1+ arterioles. (A, C) Cultured arteriole explants from TRPV1Cre/R26R-YFP mice were imaged with Fura-2-AM dye at baseline (BL) (A), and following stimulation with 10μM CAP (C). (E) EYFP expression in arteriolar SMCs of this mouse. (B, D, F) Explants from Trpv1 knockout mice lack CAP responses (D) but maintain responses to HiK (F). (G) 340/380 ratios of boxed regions in A. (H) 340/380 ratios of boxed regions in B. (I) Average responses of arterioles from TRPV1Cre and Trpv1 knockout mice following application of CAP, RR, and HiK (n = 4). ^ p < .05, ^^ p < .01 compared to BL; ** p < .01 compared to TRPV1Cre; two-way ANOVA with Bonferroni post-hoc test. Scale bar = 100 μm.

Daniel J. Cavanaugh, et al. J Neurosci. ;31(13):5067-5077.
4.
Figure 7

Figure 7. From: Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells.

TRPV1 expression in a subset arteriolar smooth muscle cells. (A) PLAP staining in cremaster muscle, showing PLAP+ (arrows), and PLAP- (arrowheads) vessels. (B) nlacZ staining in cremaster muscle. (C) PLAP staining in dura, showing PLAP+ (arrows), and PLAP- (arrowheads) vessels. (D) nlacZ in tongue (magenta) co-localizes with staining for smooth muscle actin (green). Inset shows magnification of boxed area. PLAP+ smooth muscle cells (arrows) were also observed in trachea (E), and skin (F). Arrowheads in (E-F) point to PLAP+ axonal label, most likely of primary afferent origin. (G-H) RTX eliminates nlacZ staining in the ear. (I-J) A similar pattern of lacZ staining was seen in TRPV1Cre/R26R-lacZ mice. Shown are images from tongue (I) and ear (J). In (J), arrow points to lacZ+ smooth muscle cell, and arrowhead points to lacZ+ nerve. Scale bars in A-C, E-H and J = 500 μm, in D and I = 200 μm.

Daniel J. Cavanaugh, et al. J Neurosci. ;31(13):5067-5077.
5.
Figure 1

Figure 1. From: Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells.

Primary afferent expression of reporter molecules in TRPV1PLAP-nlacZ mice. (A) Schematic showing the Trpv1 locus of TRPV1PLAP-nlacZ mice. (B) nlacZ is present in nuclei of DRG neurons. (C) PLAP is present both in cell bodies (arrowheads) and axonal processes (arrows) of DRG neurons. (D) PLAP staining in primary afferent terminals in the SC dorsal horn. (E) nlacZ staining (magenta) in DRG section shows near complete overlap with TRPV1 immunoreactivity (green). (F-H) Cultured adult DRG neurons were imaged with Fura-2-AM dye at baseline (F), and following stimulation with 1μM CAP (G), and high K+ Ringer’s solution (HiK) (H). (I) nlacZ staining in these cells. (J) 340/380 ratios of cells as numbered in I. Scale bars in B, C, E and J = 100 μm, in D = 200 μm.

Daniel J. Cavanaugh, et al. J Neurosci. ;31(13):5067-5077.
6.
Figure 6

Figure 6. From: Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells.

Functional TRPV1 expression in brain regions implicated in the TRPV1 knock-in mice. (A-H) Calcium imaging of SuM neurons in slices (A-D) and acutely dissociated cultures (E-H) from TRPV1Cre/R26R-EYFP mice during application of 10 μM CAP (B, F) and high K+ ACSF (C, G). (D, H) Endogenous EYFP fluorescence. (I-J) Representative 340/380 ratios, of the EYFP positive cells from slices (normalized to baseline) (I) and acutely dissociated neurons (J). (J, lower panel) 340/380 ratios of cells from another well, demonstrating a block of CAP-induced calcium increases by RR. Arrows in D and H point to CAP-responsive, EYPF+ cells; arrowhead points to EYFP+ cell that did not respond to CAP. (K) Whole-cell recordings of EYFP+ SuM cells in brain slices of TRPV1Cre/R26R-EYFP mice during application of 10 μM CAP Red trace shows the average response of all CAP-responsive cells. (L-N) Calcium imaging of dentate gyrus neurons in slices during application of 10 μM CAP (M) and high K+ ACSF (N). Insets are magnified images of dashed region in L. (O) Representative 340/380 ratios, normalized to baseline, of 30 dentate gyrus neurons during calcium imaging.

Daniel J. Cavanaugh, et al. J Neurosci. ;31(13):5067-5077.
7.
Figure 3

Figure 3. From: Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells.

Radioactive in situ for TRPV1 in DRG and brain of mouse, rat and human. (A) Brightfield image of radioactive in situ in mouse DRG, counterstained with H&E. (B) Double labeling with TRPV1 antibody (brown) and radioactive in situ probe (black) shows complete colocalization between the two in mouse DRG. (C) Brightfield image of radioactive in situ in rat DRG, counterstained with H&E. S, M and L indicate small, medium and large diameter neurons, respectively. * Indicates negative cell. (D) Darkfield image of radioactive in situ in rat DRG. (E-H) Coronal sections of rat brain processed for radioactive TRPV1 in situ hybridization showing positive signals in PH (E), SuM (F), IF (G), and dorsomedial motor nucleus of the vagus (10th motor nucleus; DMV) (H). Closeup images of indicated regions are shown below in (I-L) for rat and in (M-P) for mouse. (Q) Darkfield image of radioactive in situ signal in human DRG. (R-S) Representative images of radioactive in situ in human brain sections, demonstrating complete lack of staining. Scalebars = 100 μm in A, B and Q, 50 μm in C-D and I-L, 2 mm in E-H, 25 μm in M-P, and 5 mm in R-S.

Daniel J. Cavanaugh, et al. J Neurosci. ;31(13):5067-5077.
8.
Figure 5

Figure 5. From: Trpv1 reporter mice reveal highly restricted brain distribution and functional expression in arteriolar smooth muscle cells.

Developmental expression of TRPV1 in the brain. Left panels show lacZ staining in 80 μm sections of TRPV1Cre/R26R-lacZ mice. Right panels show lack of nlacZ staining in the same areas in TRPV1PLAP-nlacZ mice, likely due to developmental expression that is extinguished in the adult. Regions include the 10th and 12th motor nuclei of the medulla (A) (arrowheads indicate cell body staining in 10th and 12th motor nuclei; arrows indicate axonal lacZ label in the area postrema and NTS), nucleus interpolaris (B), perivolivary region (C), raphe magnus (D), central gray region (E), inferior colliculus (F), parabrachial (PB) nucleus (arrowheads) and nucleus of the lateral lemniscus (arrows) (G), lateral hypothalamic nucleus (H, arrowheads), and paraventricular hypothalamic nucleus (PVH) (I). (J) RT-PCR analysis of brain regions and peripheral tissues to detect presence of TRPV1 mRNA. Note faint band in caudal hypothalamus lane (arrow). OB = olfactory bulb; Co = cortex; Hi = hippocampus; AH = anterior hypothalamus; CH = caudal hypothalamus; PG = periaqueductal gray; Ce = cerebellum; SC = spinal cord; TG = trigeminal ganglion; Li = liver; Bl = bladder; Cr = cremaster; neg = no cDNA. Scale bar = 500 μm.

Daniel J. Cavanaugh, et al. J Neurosci. ;31(13):5067-5077.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk