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Results: 5

1.
Fig. 4

Fig. 4. DLM facilitates the survival of human primary hepatocytes in vivo. From: Decellularized Liver Matrix as a Carrier for Transplantation of Human Fetal and Primary Hepatocytes in Mice.

(A) GUSB staining (red) of human primary hepatocytes in the DLM 1 week after implantation into NOD/SCID/MPS VII mice. (B) Human primary hepatocytes transduced with the lentiviral LUX-PGK-EGFP vector and reconstituted in DLM were implanted into NOD/SCID/IL2rγ−/− mice. The fluorescent image of the harvested DLM was made 6 weeks after implantation. GFP-positive human primary hepatocytes were visualized in green within the DLM.

Ping Zhou, et al. Liver Transpl. ;17(4):418-427.
2.
Fig. 2

Fig. 2. FH-hTERT cultured in DLM. From: Decellularized Liver Matrix as a Carrier for Transplantation of Human Fetal and Primary Hepatocytes in Mice.

FH-hTERT transduced with a lentiviral vector carrying LUX-PGK-EGFP were infused into the DLM after the completion of perfusion (A) and cultured for 7 days (B & C). Fluorescent images were taken at 40× (A & B) and 200× (C) magnification. (D) Quantitative real-time RT-PCR analysis of ALB and AAT mRNA levels in FH-hTERT reconstituted in DLM cultured for 7 days. ** p < 0.01 compared to FH-hTERT cultured in standard conditions (n=3).

Ping Zhou, et al. Liver Transpl. ;17(4):418-427.
3.
Fig. 3

Fig. 3. Bioluminescent imaging of FH-hTERT over time after transplantation. From: Decellularized Liver Matrix as a Carrier for Transplantation of Human Fetal and Primary Hepatocytes in Mice.

After transduction with lentiviral LUX-PGK-EGFP vector and enrichment by FACS, FH-hTERT were either infused into DLM and then implanted into mice or transplanted via splenic or omentum injection. (A) Representative bioluminescent images for the same mice over time with three modes of transplantation. (B) Bioluminescent signal intensity for the mice with splenic injection (n=5), omentum injection (n=4) or DLM implantation (n=4) at each time point. *, *** and **** correspond to P<0.05, 0.005 and 0.001 respectively in comparison to splenic injection at corresponding time points. Δ and ΔΔ correspond to p<0.05 and 0.01 in comparison to omentum injection at corresponding time points. The line indicates minimal signal strength to be imaged.

Ping Zhou, et al. Liver Transpl. ;17(4):418-427.
4.
Fig. 5

Fig. 5. Quantitative real-time RT-PCR analysis of mRNA levels of the liver-specific gene. From: Decellularized Liver Matrix as a Carrier for Transplantation of Human Fetal and Primary Hepatocytes in Mice.

ALB (A), CYP3A4 (B), CYP1A1 (C) and CYP2C9 (D) in the livers or DLM implants of transplanted mice 6 weeks after transplantation. Human primary hepatocytes were either reconstituted in DLM or transplanted into in NOD/SCID/IL2rγ−/− mice via splenic injection. The median value of each group is indicated with a bar. The number of animals from each group is shown in each plot, and there was no significant statistical difference in gene expression levels between DLM implantation and splenic injection in B, C and D. Expression levels of liver-specific genes were calculated based on that of freshly isolated human primary hepatocytes.

Ping Zhou, et al. Liver Transpl. ;17(4):418-427.
5.
Fig. 1

Fig. 1. Characterization of the decellularized liver matrix (DLM). From: Decellularized Liver Matrix as a Carrier for Transplantation of Human Fetal and Primary Hepatocytes in Mice.

(A) Representative mouse liver images of color changes during an in situ decellularization process at 0, 12, 30, 60 and 120 min of perfusion with 1% SDS. (B) DLM harvested from a mouse after the completion of a decellularization procedure. (C) H&E staining of a DLM slice demonstrating no remaining cellular components (100×). (D). DLM was injected with crystal violet in agarose through the portal vain after the completion of a decellularization procedure for the visualization of remaining vasculature networks (20×). (E) Mouse liver and the DLM cryosections were immuno-stained with antibodies against the indicated extracellular matrix proteins (fibronectin, laminin and collagen IV in green) and DAPI (blue) in the mouse liver. Please note that there was no DAPI staining in the DLM on the corresponding right panels (200×).

Ping Zhou, et al. Liver Transpl. ;17(4):418-427.

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