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Results: 3

1.
Figure 1

Figure 1. 2D gels of FAP and control colonic fibroblasts.Three overlapping pH range 2D gels across pI 4—11 of FAP and control colonic fibroblasts. From: APC+/− alters colonic fibroblast proteome in FAP.

These files are also in the Supplemental Data and at our web site after publication and are searchable and hyperlink-enabled to gene ontology on line.

Bhavinkumar B. Patel, et al. Oncotarget. 2011 March;2(3):197-208.
2.
Figure 3

Figure 3. DJ-1 proteomic changes in colonic crypts. From: APC+/− alters colonic fibroblast proteome in FAP.

A. 1D gel Western blotting, B. 2D gel pH4—7 12% SDS-PAGE gels, and mass spectrometry protein identification, and C. 2D gel Western blotting experiments. The circled protein spots were DJ-1 identified by mass spectrometry. Corresponding spots were indicated by arrows in the 2D Western blotting images. For the 2D Western, Control was CP 203, FAP was SID 516.

Bhavinkumar B. Patel, et al. Oncotarget. 2011 March;2(3):197-208.
3.
Figure 2

Figure 2. RSU-1 expression in colonic fibroblasts. From: APC+/− alters colonic fibroblast proteome in FAP.

A. pH 6—11 range 2D gel analysis of RSU-1 protein isoforms in control colon fibroblasts. All the protein spots observed in this picture were identified with mass spectrometry. Four isoforms of RSU-1 are indicated by the arrows. The rightmost spot is unmodified RSU-1. The second spot from the left is lower in intensity in FAP fibroblasts. The right two RSU-1 spots were outside of the range of the pH 5—8 gels previously used to detect changes in RSU-1 levels. B. Western analysis of RSU1 expression in FAP colonic fibroblasts versus control colonic fibroblasts. C. Western analysis of RSU-1 expression in FAP colonic crypt cells versus those from control patients.

Bhavinkumar B. Patel, et al. Oncotarget. 2011 March;2(3):197-208.

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