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1.
Figure 5

Figure 5. Confocal microscopy images showing binding of fluorescence-labeled peptide to colon adenomas.. From: In Vivo Fluorescence-Based Endoscopic Detection of Colon Dysplasia in the Mouse Using a Novel Peptide Probe.

Biopsies were taken after in vivo administration via the endoscope of the FITC-Ahx-QPIHPNNM target peptide (A) or the FITC-Ahx-GGGAGGGA control peptide (B) and subsequently imaged. Scale bar, 25 µm.

Sharon J. Miller, et al. PLoS One. 2011;6(3):e17384.
2.
Figure 3

Figure 3. Representative histology from (A) normal colonic mucosa, (B) hyperplastic Kras, and (C–D) adenoma in CPC;Apc mice.. From: In Vivo Fluorescence-Based Endoscopic Detection of Colon Dysplasia in the Mouse Using a Novel Peptide Probe.

C: An advanced dysplastic adenoma >1 mm in size. D: Higher magnification (scale bar 50 µm) of dysplasia (boxed region of C) displaying enlarged nuclei, hyperchromaticity and distorted crypts.

Sharon J. Miller, et al. PLoS One. 2011;6(3):e17384.
3.
Figure 4

Figure 4. Quantitative analysis of peptide adsorption to distal colonic adenomas showing target peptide FITC-Ahx-QPIHPNNM (2.17±0.61, n = 18) binds greater to the adenomas when compared to the control peptide FITC-Ahx-GGGAGGGA (1.14±0.15, n = 7).. From: In Vivo Fluorescence-Based Endoscopic Detection of Colon Dysplasia in the Mouse Using a Novel Peptide Probe.

The mean of each group is represented by a horizontal black line with one standard deviation from the mean displayed. Non-parametric Mann-Whitney independent samples analysis demonstrates target peptide binds more significantly than control, p<0.01.

Sharon J. Miller, et al. PLoS One. 2011;6(3):e17384.
4.
Figure 1

Figure 1. Titer results showing plaque forming units (pfu) for adenomas normalized to the number of phages bound to normal colonic mucosa.. From: In Vivo Fluorescence-Based Endoscopic Detection of Colon Dysplasia in the Mouse Using a Novel Peptide Probe.

After two rounds of panning, the recovered phage pool was first cleared against a homogenized mixture of organs (heart, kidney, liver, normal colon) and subsequently cleared against normal colon tissue only. Clearing decreased the number of phage that bound to normal colon tissue 9× or 3× in rounds 3 and 4, respectively. The titer was performed in triplicate.

Sharon J. Miller, et al. PLoS One. 2011;6(3):e17384.
5.
Figure 2

Figure 2. Images from wide-field endoscopy videos after application of fluorescence-labeled peptides.. From: In Vivo Fluorescence-Based Endoscopic Detection of Colon Dysplasia in the Mouse Using a Novel Peptide Probe.

The left and right columns represent frames from white light and fluorescence, respectively. The fluorescent labeled target peptide FITC-Ahx-QPIHPNNM showed positive binding to (A) multiple adenomas and (B) single adenoma in a CPC;Apc mouse. (C) The control peptide FITC-Ahx-GGGAGGGA showed minimal binding. The target peptide also showed minimal binding to (D) the lumen of a CPC;Apc bred mouse negative for Cre recombinase (control litter mate) and (E) the hyperplastic epithelium in a mutant Kras mouse model. White arrows identify adenomas.

Sharon J. Miller, et al. PLoS One. 2011;6(3):e17384.
6.
Figure 6

Figure 6. Wide-field and confocal images of excised adenomas incubated with peptide ex vivo.. From: In Vivo Fluorescence-Based Endoscopic Detection of Colon Dysplasia in the Mouse Using a Novel Peptide Probe.

(A) Two polyps visualized endoscopically ex vivo under conventional white light illumination. (B) Ex vivo study shows preferential binding of the fluorescent-labeled target peptide FITC-Ahx-QPIHPNNM compared to the control peptide FITC-Ahx-GGGAGGGA on excised colon. Corresponding representative confocal microscopy images showing increased binding to the adenoma incubated with (C) target peptide when compared to (D) the control. A target to control peptide binding ratio was calculated as 2.51±0.59 (s.d.) for the excised adenoma pairs. Scale bar, 25 µm.

Sharon J. Miller, et al. PLoS One. 2011;6(3):e17384.

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