We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 4

1.
Figure 1

Figure 1. From: Apurinic/Apyrimidinic Endonuclease 1 Regulates Inflammatory Response in Macrophages.

Effect of E3330 on LPS-induced production of pro-inflammatory cytokines in RAW264.7 cells. (A) TNF-α, (B) IL-6 (C) IL-12 production in cell culture media from RAW264.7 cells pretreated with E3330 for 24 hours followed by LPS for an additional 24 hours. Each value is the mean±S.D. of two independent experiments, repeated minimally twice. *p<0.05 control vs. LPS alone, *p<0.05 LPS vs. LPS plus E3330.

ANDREJ JEDINAK, et al. Anticancer Res. ;31(2):379-385.
2.
Figure 3

Figure 3. From: Apurinic/Apyrimidinic Endonuclease 1 Regulates Inflammatory Response in Macrophages.

Effect of E3330 on NF-κB and AP-1 DNA-binding. RAW264.7 cells were pretreated with E3330 for 24 hours followed by the LPS (1 μg/ml) treatment for 30 min. Nuclear extracts were subjected to gel shift analysis with (A) [32P]-labeled NF-κB probe or [32P]-labeled AP-1 probe. The specificity was confirmed by the competitive gel shift in LPS-stimulated cells with cold NF-κB or AP-1 or unrelated DNA (URL). The data are representative of three separate experiments.

ANDREJ JEDINAK, et al. Anticancer Res. ;31(2):379-385.
3.
Figure 2

Figure 2. From: Apurinic/Apyrimidinic Endonuclease 1 Regulates Inflammatory Response in Macrophages.

Effect of E3330 on LPS-induced PGE2, NO, COX-2 and iNOS expression in RAW264.7 cells. RAW264.7 cells were pretreated with E3330 for 24 hours followed by LPS for 24 hours. (A) PGE2 and (B) NO in cell culture media. Each value is the mean±S.D. of two independent experiments, repeated minimally twice. p<0.05 control vs. LPS alone, *p<0.05 LPS vs. LPS plus E3330. (C) COX-2 and (D) iNOS determined in lysates by Western blot analysis. The equal protein loading was verified with anti-β-actin antibody. The results are representative of three separate experiments.

ANDREJ JEDINAK, et al. Anticancer Res. ;31(2):379-385.
4.
Figure 4

Figure 4. From: Apurinic/Apyrimidinic Endonuclease 1 Regulates Inflammatory Response in Macrophages.

Schematic illustration of the relationship of affected molecules and pathways following inhibition of Ref-1 (APE1) redox function with E3330. Blocking Ref-1 with the small molecule Ref-1 redox inhibitor E3330 inhibits NF-κB function and most likely other transcription factors that are downstream of Ref-1 such as AP-1 and CREB leading to a decrease in synthesis of COX2, IL-12, IL-6, TNF-α and iNOS. Decreased levels of iNOS result in decreased NO produced. Decreased production of COX2 leads to fewer AA being converted by COX2 and eventually to PGE2 which could result in a signaling cascade decrease that further suppresses iNOS production via CREB suppression. NFAT, Nuclear factor of activated T-cells; PPAR, peroxisome proliferator-activated receptor; CREB, cAMP response element-binding; PR, prostagladin receptors; C/EBP, CCAAT-enhancer binding proteins. The red lettering signifies molecules measured in the present study.

ANDREJ JEDINAK, et al. Anticancer Res. ;31(2):379-385.

Supplemental Content

Recent activity

Your browsing activity is temporarily unavailable.

Write to the Help Desk