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Results: 7

1.
Figure 6

Figure 6. From: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells.

Viability comparison of CHO-S cells grown in fresh media and exposed to nutrient-depleted media with and without mmu-miR-466h inhibition.

Aliaksandr Druz, et al. Biotechnol Bioeng. ;108(7):1651-1661.
2.
Figure 1

Figure 1. From: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells.

Growth and glucose depletion of CHO cells. (A)Fresh media growth, (B) Viability comparison

Aliaksandr Druz, et al. Biotechnol Bioeng. ;108(7):1651-1661.
3.
Figure 2

Figure 2. From: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells.

Caspase-3/7 activity in CHO cells exposed to nutrient-depleted media. Assay was read continuously for 40 min in 10 min intervals.

Aliaksandr Druz, et al. Biotechnol Bioeng. ;108(7):1651-1661.
4.
Figure 3

Figure 3. From: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells.

Map of the overlaid images of the respective microRNA fluorescent signals in nutrient-depleted and fresh media grown CHO-S cells. Each well represents a distinct microRNA. Depleted media derived RNA sample was labeled with Cy3 (green) and fresh media with Cy5 (red). The wells with probes for mmu-miR-466h and mmu-miR-669c are pointed at with arrows.

Aliaksandr Druz, et al. Biotechnol Bioeng. ;108(7):1651-1661.
5.
Figure 4

Figure 4. From: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells.

qRT-PCR comparison of mmu-miR-466h, mmu-miR-669c, and mRNA levels for mmu-miR-466h predicted targets in fresh and depleted media after 24h. (A) Mmu-miR-466h and mmu-miR-669c levels. The TaqMan microRNA assays were used for both miRs with sno202 and let-7c as controls for 2−ΔΔCt analysis. (B) mRNA levels for mmu-miR-466h predicted gene targets. Respective TaqMan mRNA assays were used to assess mRNA levels with 18S as control for 2−ΔΔCt analysis.

Aliaksandr Druz, et al. Biotechnol Bioeng. ;108(7):1651-1661.
6.
Figure 5

Figure 5. From: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells.

Relative levels of mmu-miR-466h and its target genes in fresh and nutrient-depleted media with and without mmu-miR-466h inhibition. (A) Mmu-miR-466h levels. TaqMan microRNA qRT-PCR analysis was used to assess mmu-miR-466h levels at 23.5h in different media conditions with let-7c as control for 2−ΔΔCt analysis. (B) Relative mRNA levels of bcl2l2, birc6, dad1, stat5a, and smo genes in depleted media with and without mmu-miR-466h inhibition based on TaqMan qRT-PCR data. 18S levels were used as control for 2−ΔΔCt analysis.

Aliaksandr Druz, et al. Biotechnol Bioeng. ;108(7):1651-1661.
7.
Figure 7

Figure 7. From: A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells.

Comparison of Caspase-3/7 activity at different time points during fresh media incubation and nutrient-depleted media incubation with and without mmu-miR-466h inhibition. Depleted media incubated CHO cells were transfected with anti-miR-466h or anti-miR negative control 22h before depleted media exposure. Caspase plate was read continuously for 60 min in 15 min intervals. (A) 17h incubation, (B) 18.5h incubation, (C) 20h incubation.

Aliaksandr Druz, et al. Biotechnol Bioeng. ;108(7):1651-1661.

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