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Figure 1

Figure 1. From: Adipose Derived Stromal Cells for Skeletal Regenerative Medicine.

In vivo model for skeletal tissue engineering.(Left, Top) Superior view of mouse left inguinal fat pad. Red arrow points to the fat pad which is dissected and subsequently digested for ASC harvest. (Left, Bottom) Human lipoaspirate in sterile collection canister settled into two layers. The upper yellow layer (Green Arrow) is the adipose tissue from which ASCs are harvested. Subsequently ASCs are isolated in vitro. 150,000 cells can then be seeded on an osteoconductive scaffold (Middle) and then the ASC laden scaffold can be placed inside a critical sized calvarial defect in syngeneic for mASCs or nude athymic mice for hASCs (Right). Mice can then be imaged using IVIS systems to detect cell viability and MicroCT to quantify osseous healing (Right).

Benjamin Levi, et al. Stem Cells. ;29(4):576-582.

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