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1.
Figure 1

Figure 1. From: Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis.

Mean protein concentration of skimmed milks from C57BL/6J and SEG/Pas mice, at 10 days of lactation. The number of mice used in each population is given in parentheses. Error bars correspond to the standard error.

Nisrine Boumahrou, et al. BMC Genomics. 2011;12:80-80.
2.
Figure 3

Figure 3. From: Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis.

2-DE of C57BL/6J and SEG/Pas skimmed milks at 10 days of lactation. The protein spots showed by arrows were identified by a PMF analysis as being Serum Albumin (SA), αs1-casein (αs1), β-casein (β), γ-casein (γ), Whey Acidic Protein (WAP) and α-lactalbumin (α-lac).

Nisrine Boumahrou, et al. BMC Genomics. 2011;12:80-80.
3.
Figure 4

Figure 4. From: Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis.

SDS-PAGE analysis of skimmed milks collected from C57BL/6J and SEG/Pas mice at 4, 8, and 14 days of lactation. Names of the main proteins are indicated (Lf: Lactoferrin, SA: Serum Albumin, αs1: αs1-casein, β: β-casein, γ: γ-casein, ε: ε-casein, WAP: Whey Acidic Protein). MM: Molecular markers.

Nisrine Boumahrou, et al. BMC Genomics. 2011;12:80-80.
4.
Figure 7

Figure 7. From: Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis.

Alignment of nucleotide and amino acid sequences of C57BL/6J and SEG/Pas Wap. The deduced amino acid sequence is given above according to the one-letter code. Horizontal arrows indicate the position and orientation of primers used for amplification. Pink frames show the SNP between C57BL/6J and SEG/Pas and blue frames indicate the translation-initiation and termination codon. Red amino acid residues in SEG/Pas sequence represent amino acid substitutions (K36E, T94A, M99K); Δ represents the loss of the serine in position 93.

Nisrine Boumahrou, et al. BMC Genomics. 2011;12:80-80.
5.
Figure 6

Figure 6. From: Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis.

Alignment of the amino acid sequence of β-casein as deduced from the cDNA obtained from C57BL/6J and SEG/Pas. Peptides sequences are split into blocks of amino acid residues to visualize the exonic modular structure of the protein as deduced from known splice junctions of the Mus musculus sequence. Red amino acids in C57BL/6J sequence indicate the amino acid substitutions in SEG/Pas sequence. A* depicts a conservative mutation in the corresponding codon (GCC-> GCT in M. spretus). The pink vertical arrow indicates the deletion of a Q residue arising in the 6th exon, during the course of the splicing process in C57BL/6J and SEG/Pas mouse strains.

Nisrine Boumahrou, et al. BMC Genomics. 2011;12:80-80.
6.
Figure 8

Figure 8. From: Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis.

Schematic representation of the genomic region spanning exon 14 to exon 18 of the gene encoding αs1-casein in cow and mouse. Diagonally hatched block and segment in the cow genome indicate exon 16 and a part of the downstream intron which is first duplicated in the mouse genome (pink arrow). This first duplication (yellow block) is duplicated in tandem seven times in the mouse genome (blue arrow). The green block indicates exon 15 existing only in cattle, sheep and goats. The red intronic segment (30 nucleotides) appears to be conserved between several species (cattle, mice, pigs and humans). In addition in mice, there is a 350-nucleotides insertion between exon 16.14 and 17, contributing to enlarge this genome segment, as compared with cow (3 kb vs. 2 kb).

Nisrine Boumahrou, et al. BMC Genomics. 2011;12:80-80.
7.
Figure 2

Figure 2. From: Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis.

RP-HPLC elution profiles of C57BL/6J (green) and SEG/Pas (blue) skimmed milks samples at 10 days of lactation. Clarified milk samples (100 μl; ca. 350 μg of proteins) were injected on a Jupiter C5 Reverse Phase column (300 Å pore size, 5 μm, 150 × 4.6 mm; Phenomenex, Paris, France). The elution was achieved by a two steps linear gradient: 100% A (TFA/H2O 1.1:1000 v/v) to 20% solvent B (TFA/CAN 1:1000 v/v) over 10 min followed by 37% solvent B to 60% over 45 min at a flow rate of 1ml/min. The column was kept at 40°C. Proteins showing a different behavior between mouse species are indicated in pink.

Nisrine Boumahrou, et al. BMC Genomics. 2011;12:80-80.
8.
Figure 5

Figure 5. From: Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis.

Schematic representation of the intron/exon structure organization of cow CSN1S1 and mouse Csn1s1 genes (panel A) and of the corresponding matured transcripts (panel B) found in the mammary tissue of C57BL/6J and SEG/Pas mouse strains. Open bars represent introns; exons are depicted by large black (3'and 5'untranslated regions) or grey (exons encoding preproteins) boxes, hatched blocks indicate potentially skipped sequence, pink and yellow blocks refer to repeats of exon 16 and no-fill blocks represent exons that are constitutively absent from mRNA according to [29,37]. Numbers above blocks indicate exon number; numbers below (italics) indicate exon size (in bp). Vertical green arrows indicate exons (or sequences) skipped (or included) during the course of the splicing process. C57BL/6J: a, deletion of the first codon of exon 8 and of the first 57 nucleotides of exon 21, b: exon-skipping of exon 9 and 10, c: exon-skipping of exon 16.3. SEG/Pas: a', deletion of the first 57 nucleotides of exon 21; b', deletion of the first codon of exon 8 and exon-skipping of exons 9, 16.14 and 17; c', insertion of the last 33 nucleotides of intron 10.

Nisrine Boumahrou, et al. BMC Genomics. 2011;12:80-80.

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