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1.
Fig. 3

Fig. 3. From: Development of a versatile two-photon endoscope for biological imaging.

Two-photon images of fluorescent spheres with diameters of 10 μm (a), 3.0-3.4μm (b), and 1μm (c), obtained with the endoscope. Scale bars are 20 μm.

Youbo Zhao, et al. Biomed Opt Express. 2010 November 1;1(4):1159-1172.
2.
Fig. 7

Fig. 7. From: Development of a versatile two-photon endoscope for biological imaging.

Two-photon images of 3.0-3.4 μm fluorescent beads obtained with the endoscope before (a) and after (b) removal of the center distortions. (c) and (d) are the waveforms used to drive the PZT to generate images (a) and (b), respectively.

Youbo Zhao, et al. Biomed Opt Express. 2010 November 1;1(4):1159-1172.
3.
Fig. 6

Fig. 6. From: Development of a versatile two-photon endoscope for biological imaging.

Scanning pattern of the laser focus obtained by applying constant amplitude sinusoidal waveforms to the two channels of the PZT. The relative phases between the two drive signals are (a) 90° and (b) 50°.

Youbo Zhao, et al. Biomed Opt Express. 2010 November 1;1(4):1159-1172.
4.
Fig. 1

Fig. 1. From: Development of a versatile two-photon endoscope for biological imaging.

Schematic setup of the two-photon excitation fluorescence endoscope (a), structure of the piezoelectric actuator (b), drive waveform for a spiral scan of the fiber distal tip (c), and photograph of the assembled 2P microscope probe (d).

Youbo Zhao, et al. Biomed Opt Express. 2010 November 1;1(4):1159-1172.
5.
Fig. 8

Fig. 8. From: Development of a versatile two-photon endoscope for biological imaging.

Two-photon endoscopic images from a sequence of human TM z-sections. The depth interval of successive images is 20 μm. Scale bars are 20 μm. Top images show irregularly arranged strands in the uveal meshwork, and bottom images display flat and interacting beams or plates in the corneoscleral meshwork. Nuclei of TM cells were stained with DAPI.

Youbo Zhao, et al. Biomed Opt Express. 2010 November 1;1(4):1159-1172.
6.
Fig. 5

Fig. 5. From: Development of a versatile two-photon endoscope for biological imaging.

Calculated chromatic effects of different lens configurations: (a) shift in the focal point with respect to the fiber tip and (b) change in the fluorescence collection efficiency as functions of fluorescence wavelength. The solid, dashed, and dotted curves correspond to the three doublet lens, single GRIN lens, and GRIN + aspheric lens configurations, respectively.

Youbo Zhao, et al. Biomed Opt Express. 2010 November 1;1(4):1159-1172.
7.
Fig. 2

Fig. 2. From: Development of a versatile two-photon endoscope for biological imaging.

Schematic drawing of the three lens configurations. O: Objective plane (fiber end surface); P1: objective principle plane; P2: image principle plane; I: image plane. (a), L: 0.25 pitch GRIN lens; (b), L1: 0.15 NA aspheric lens, L2: 0.23 pitch GRIN lens; (c), L1: doublet lens with focal length of 6 mm; L2, L3: doublet lenses with focal length of 3 mm.

Youbo Zhao, et al. Biomed Opt Express. 2010 November 1;1(4):1159-1172.
8.
Fig. 4

Fig. 4. From: Development of a versatile two-photon endoscope for biological imaging.

Fluorescence intensity distributions (open circles) of a 1μm fluorescent bead along the x (panel a) and z (panel b) directions, measured with the endoscope. The full width at half maximum (FWHM) values of the fitted Gaussian curves (solid red traces passing through the points) are 1.5 μm and 9.2 μm, which indicate the lateral and axial resolutions of the system, respectively. The computed point spread functions (solid blue curves), and the convolutions (black dashes) of the point spread functions and the beads profiles show the theoretical simulations.

Youbo Zhao, et al. Biomed Opt Express. 2010 November 1;1(4):1159-1172.

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