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1.
FIGURE 1.

FIGURE 1. From: Relationship between Prion Propensity and the Rates of Individual Molecular Steps of Fibril Assembly.

Sequence alignment of Ure2p from S. cerevisiae and S. paradoxus. The differences in amino acid sequence between the two homologues all lie within the N/Q-rich N-terminal prion domain (boxed area), while the C-domains are identical.

Yi-Qian Wang, et al. J Biol Chem. 2011 April 8;286(14):12101-12107.
2.
FIGURE 3.

FIGURE 3. From: Relationship between Prion Propensity and the Rates of Individual Molecular Steps of Fibril Assembly.

Mechanism of amyloid fibril formation. For seeded growth, when nucleation kn can be neglected, there are two kinetic constants defining the growth kinetics: the elongation rate constant (k+) and the breakage rate constant (k). A, spontaneous fibril formation of SpUre2p. B, fibril formation of SpUre2p under the induction of Sc seeds.

Yi-Qian Wang, et al. J Biol Chem. 2011 April 8;286(14):12101-12107.
3.
FIGURE 4.

FIGURE 4. From: Relationship between Prion Propensity and the Rates of Individual Molecular Steps of Fibril Assembly.

Electron microscopy images of Sc seeds and Sp seeds, and the fibrils produced in the cross-seeding experiments. The images show (A) freshly prepared seeds, (B) self-seeded fibrils, and (C) cross-seeded fibrils grown in a solution of 20 μm ScUre2p (left column) or SpUre2p (right column) in 50 mm Tris-HCl, pH 8.4, 200 mm NaCl, as described in the “Experimental Procedures.” The bars represent 200 nm.

Yi-Qian Wang, et al. J Biol Chem. 2011 April 8;286(14):12101-12107.
4.
FIGURE 5.

FIGURE 5. From: Relationship between Prion Propensity and the Rates of Individual Molecular Steps of Fibril Assembly.

Time courses of fibril formation in cross-seeding experiments monitored by ThT fluorescence. Solutions (20 μm) of ScUre2p (A and B) or SpUre2p (C and D) were incubated with a series of concentrations of Sc seeds (A and C) or Sp seeds (B and D): 0% seed (brown), 1% seed (orange), 2% seed (ochre), 4% seed (green), 7% seed (teal), 10% seed (blue), in 50 mm Tris-HCl, pH 8.4, 200 mm NaCl. The data were fitted globally to obtain the ratios of the rate constants for elongation and breakage, as described under “Experimental Procedures.”

Yi-Qian Wang, et al. J Biol Chem. 2011 April 8;286(14):12101-12107.
5.
FIGURE 2.

FIGURE 2. From: Relationship between Prion Propensity and the Rates of Individual Molecular Steps of Fibril Assembly.

Linear relationship between ThT intensity and protein concentration in fibrillar form. A and B, different concentrations of seeds were produced by serial dilution of (A) ScUre2p fibrils and (B) SpUre2p fibrils, and the ThT intensity was plotted against the relative seed concentration. C and D, relationship of ThT intensity and concentration of Ure2p in the pellet fraction at different stages throughout the process of fibril formation for (C) ScUre2p and (D) SpUre2p. The data from 5 growth curves were included for each protein. The points within the plateau region (where the error in determining the protein concentration is largest) were averaged for each individual growth curve, and the error bars represent the standard error of the mean (n = 3 to 6).

Yi-Qian Wang, et al. J Biol Chem. 2011 April 8;286(14):12101-12107.
6.
FIGURE 6.

FIGURE 6. From: Relationship between Prion Propensity and the Rates of Individual Molecular Steps of Fibril Assembly.

Measurement of fibril growth kinetics using QCM. Cross-seeding experiments were performed using (A) Sc seeds or (B) Sp seeds immobilized on the QCM Chip in 50 mm Tris-HCl, pH 8.4, 200 mm NaCl. The linear decline in frequency of the piezoelectric quartz crystal was detected when solutions of ScUre2p (red line) or SpUre2p (black line) were introduced into the flow cell. C, AFM images of QCM chips before and after fibril growth. The seeds immobilized on the QCM sensor (left) and the fibrils obtained after the growth experiment (right) were observed by tapping mode AFM. A 4-μm square area was scanned in each case.

Yi-Qian Wang, et al. J Biol Chem. 2011 April 8;286(14):12101-12107.

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