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Results: 5

1.
Figure 5

Figure 5. From: Phenotypic variations in NF1-associated low grade astrocytomas: possible role for increased mTOR activation in a subset.

Increased frequency of combined phospho-S6/phospho-S6 kinase is present in low grade astrocytomas subtype indeterminate (LGSI). Using immunohistochemistry for phospho-S6 and phospho-S6 kinase, combined immunoreactivity was present in all LGSI tested (4), but in less than half of PA.

Mark Jentoft, et al. Int J Clin Exp Pathol. 2011 January 1;4(1):43-57.
2.
Figure 4

Figure 4. From: Phenotypic variations in NF1-associated low grade astrocytomas: possible role for increased mTOR activation in a subset.

Increased phospho-S6 levels are a feature of low grade astrocytomas subtype indeterminate (LGSI). One case of LGSI tested showed a strong band when using an anti phospho-S6 antibody (A). When quantifying relative band intensity using Im-ageJ software, there was 12-40% increased levels in the LGSI compared to two NF1-associated PA. Levels were adjusted for total S6 to control for gel loading. Cbll=cerebellum, GBM=Glioblastoma sample control, cortex=non-neoplastic cerebral cortex.

Mark Jentoft, et al. Int J Clin Exp Pathol. 2011 January 1;4(1):43-57.
3.
Figure 3

Figure 3. From: Phenotypic variations in NF1-associated low grade astrocytomas: possible role for increased mTOR activation in a subset.

Low grade astrocytomas subtype indeterminate (LGSI) express neuronal associated proteins. A subset of LGSI show peculiar morphologic features including plump, eosinophilic cytoplasmic processes and macronucleoli (A) (H&E). Strong expression of GFAP was noted in all tumors (B). Synaptophysin immunoreactivity, sometimes in a paranuclear pattern in addition to cytoplasmic/cell surface pattern, was noted in half of the cases tested (C). Immunoreactivity for neurofilament protein in isolated cells was also a feature of synaptophysin expressing tumors (D).

Mark Jentoft, et al. Int J Clin Exp Pathol. 2011 January 1;4(1):43-57.
4.
Figure 1

Figure 1. From: Phenotypic variations in NF1-associated low grade astrocytomas: possible role for increased mTOR activation in a subset.

Low grade astrocytomas subtype indeterminate (LGSI) demonstrate ultrastructural features of glial as well as neuronal differentiation. LGSI tumors demonstrated conventional features of glial differentiation including glial intermediate filaments and electrodense Rosenthal fiber material. In addition aligned microtubules and dense core granules were present, consistent with partial neuronal differentiation. Ultrastructural characteristics of neuronal differentiation were infrequent in conventional pilocytic astrocytomas (PA) and absent in the diffuse astrocytoma (DA). Curiously, the latter also showed conspicuous intracytoplasmic Rosenthal fiber material (arrows: Rosenthal fiber material; arrowheads: dense core granules; EGB: eosinophilic granular bodies, Fil=intermediate filaments; MTs: aligned microtubules).

Mark Jentoft, et al. Int J Clin Exp Pathol. 2011 January 1;4(1):43-57.
5.
Figure 2

Figure 2. From: Phenotypic variations in NF1-associated low grade astrocytomas: possible role for increased mTOR activation in a subset.

A subset of NF1-associated low grade astrocytomas show distinctive morphology. The subset of low grade astrocytomas characterized by plump processes and macronucleoli had a predilection for the supratentorial brain hemispheres, where they formed well circumscribed masses (A). Corresponding histology (B), a tumor that also expressed chromogranin A in isolated cells (inset). Another example of LGSI (case used for gene expression and Western Blot studies in figure 4 and table 3)(C). These tumors demonstrated some overlap with pilocytic astrocytoma, including the presence of elongated bipolar cells in a loose textured background (D), but lacked Rosenthal fibers at the H&E level in all instances, and eosinophilic granular bodies in most.

Mark Jentoft, et al. Int J Clin Exp Pathol. 2011 January 1;4(1):43-57.

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