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Results: 6

1.
Figure 6.

Figure 6. From: Reverse cholesterol transport is elevated in carboxyl ester lipase-knockout mice.

Hepatic cholesterol synthesis is higher in Cel−/− mice. 15 mCi of [3H]2O was injected into mice midway through the dark cycle, and digitonin-precipitable radiolabel in liver was quantitated after 1 h, as described in Materials and Methods; n = 5 control and 6 Cel−/− mice. *P = 0.027.

Lisa M. Camarota, et al. FASEB J. 2011 April;25(4):1370-1377.
2.
Figure 3.

Figure 3. From: Reverse cholesterol transport is elevated in carboxyl ester lipase-knockout mice.

Excretion of HDL-CE is increased in Cel−/− mice, and the sterol remains esterified. Total fecal output was collected for 24 h after tail vein injection of radiolabeled HDL-[3H]CE. Neutral sterols were extracted, and radiolabel in free vs. esterified cholesterol was resolved by TLC and quantitated; n = 6 control and 4 Cel−/− mice. *P < 0.006 vs. controls.

Lisa M. Camarota, et al. FASEB J. 2011 April;25(4):1370-1377.
3.
Figure 4.

Figure 4. From: Reverse cholesterol transport is elevated in carboxyl ester lipase-knockout mice.

Total mass of fecal sterols is greater for Cel−/− mice, including increased mass of esterified cholesterol. A) Neutral and acidic sterols were extracted from feces and quantitated as described in Materials and Methods; n = 8 control and 8 Cel−/− mice. *P < 0.001; #P = 0.034. B) Neutral sterols were extracted from feces without saponification and resolved by TLC to distinguish free and esterified cholesterol. FC, free cholesterol; CE, esterified cholesterol (loaded as markers in outside lanes).

Lisa M. Camarota, et al. FASEB J. 2011 April;25(4):1370-1377.
4.
Figure 5.

Figure 5. From: Reverse cholesterol transport is elevated in carboxyl ester lipase-knockout mice.

RCT of macrophage cholesterol is greater in Cel−/− mice. J774 mouse macrophage cells were loaded with radiolabeled acetylated LDL ([3H]cholesteryl oleate) and given to mice by i.p. injection. Total fecal output was collected for the subsequent 3 d, and radiolabel in neutral (A) and acidic (B) sterols was quantitated as described in Materials and Methods; n = 8 control and 10 Cel−/− mice. *P < 0.001, #P ≤ 0.023.

Lisa M. Camarota, et al. FASEB J. 2011 April;25(4):1370-1377.
5.
Figure 2.

Figure 2. From: Reverse cholesterol transport is elevated in carboxyl ester lipase-knockout mice.

CE content of gall bladder bile is greater in Cel−/− mice. Gall bladder (GB) bile was collected midway through the light cycle. A) Equal volumes of bile from control and Cel−/− mice were resolved by TLC and visualized with phosphomolybdic acid. CE is clearly seen in Cel−/− bile but much less in control bile. B) Free and esterified cholesterol levels were determined in other samples by direct enzymatic assay, as described in Materials and Methods; n = 8 control and 13 Cel−/− mice. *P ≤ 0.02 vs. controls.

Lisa M. Camarota, et al. FASEB J. 2011 April;25(4):1370-1377.
6.
Figure 1.

Figure 1. From: Reverse cholesterol transport is elevated in carboxyl ester lipase-knockout mice.

Reduced hydrolysis of HDL-CE but increased transport to hepatic bile in Cel−/− mice. A) Flowing hepatic bile was collected for 1 h after injection of HDL-[3H]CE into the vena cava of anesthetized mice. Lipids were resolved by TLC to separate cholesterol from CE, and the amount of radiolabel in each band was quantitated. Data are presented as the percentage of radiolabeled biliary cholesterol that was hydrolyzed; n = 4 control and 7 Cel−/− mice. *P < 0.0001. B) Total counts transported from HDL-[3H]CE into hepatic bile were determined after 2 h of collection. Data are expressed as percentage of injected dose; n = 4 each. *P = 0.020.

Lisa M. Camarota, et al. FASEB J. 2011 April;25(4):1370-1377.

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