Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 8

1.
FIG. 4.

FIG. 4. From: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy .

Histogram of the spacing values obtained from the AdG model.

Ivan E. Ivanov, et al. J Bacteriol. 2011 March;193(5):1259-1266.
2.
FIG. 5.

FIG. 5. From: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy .

LPS layer compressibility (δ offset) histogram. DΔM and Wzy::GM are the most compressible, followed, respectively, by ΔWzz2, wild-type PA103, and ΔWzz1.

Ivan E. Ivanov, et al. J Bacteriol. 2011 March;193(5):1259-1266.
3.
FIG. 2.

FIG. 2. From: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy .

Representative approach curves obtained from the PA103 wild-type strain and the four LPS mutants. Repulsive forces between the sample and the AFM tip increase with increasing LPS length.

Ivan E. Ivanov, et al. J Bacteriol. 2011 March;193(5):1259-1266.
4.
FIG. 6.

FIG. 6. From: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy .

Box plot of adhesion data obtained from AFM experiments. Wild-type strain PA103 shows the highest average force, while all LPS mutations lower the force of adhesion to the Si3N4 tip. Of the four mutants, DΔM shows the highest absolute force.

Ivan E. Ivanov, et al. J Bacteriol. 2011 March;193(5):1259-1266.
5.
FIG. 7.

FIG. 7. From: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy .

Histogram of force data obtained from AFM experiments. PA103 shows the broadest distribution range, while the force values obtained from ΔWzz1, ΔWzz2, and Wzy::GM show similar Gaussian distribution patterns. The frequency along the vertical axis ranges from 0 to 100% for each of the treatments.

Ivan E. Ivanov, et al. J Bacteriol. 2011 March;193(5):1259-1266.
6.
FIG. 8.

FIG. 8. From: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy .

Survival of mice infected with wild-type PA103 and the ΔWzz mutants. The doses used for infection were as follows: PA103, 6.8 × 105 CFU; ΔWzz1, 7.5 × 105 CFU; ΔWzz2, 6.7 × 105 CFU. Four mice were included per group. Curves were plotted using the Kaplan-Meier method, and curve comparisons were done using the log-rank test. The PA103 and ΔWzz1 survival curves were significantly different from each other (P < 0.05), while the PA103 and ΔWzz2 curves were not significantly different.

Ivan E. Ivanov, et al. J Bacteriol. 2011 March;193(5):1259-1266.
7.
FIG. 3.

FIG. 3. From: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy .

Histogram showing the length distribution of the LPSs of PA103 and the four mutants. DΔM and Wzy::GM show the shortest lengths resulting from interactions with A-band LPS molecules. Following is ΔWzz2 with the long O-antigen side chain preserved. The wild type and ΔWzz1 (lacking long O-antigen side chains but still possessing the very long ones) express LPSs of the greatest length.

Ivan E. Ivanov, et al. J Bacteriol. 2011 March;193(5):1259-1266.
8.
FIG. 1.

FIG. 1. From: Relating the Physical Properties of Pseudomonas aeruginosa Lipopolysaccharides to Virulence by Atomic Force Microscopy .

Western blot assay of LPSs isolated from P. aeruginosa PA103 and mutants. Isolated LPS was separated by either 8% (A) or 12% (B and C) SDS-PAGE and immunoblotted using (A) polyclonal P. aeruginosa serotype O11 antibody on purified LPS, (B) monoclonal antibody to the A-band common antigen, and (C) monoclonal antibody to serotype O11. Wild-type PA103 produces both long and very long B-band O-antigen side chains, while ΔWzz1 and ΔWzz2 express only very long and long LPS chains, respectively. Wzy::GM has only one O-antigen subunit attached to the lipid A-core (13).

Ivan E. Ivanov, et al. J Bacteriol. 2011 March;193(5):1259-1266.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk