Results: 4

1.
Fig. 3.

Fig. 3. From: Genetic immunization in the lung induces potent local and systemic immune responses.

Cellular immunity induced by aerosolized rAd35 immunization. (A) rAd35 encoding the TB antigens 85A, 85B, and 10.4 was given by 4-μm aerosol administration to four animals at three different doses and to three animals by i.m. injection (1011 particles). Animals were immunized at week 0 and week 8. Shown are the 85A/B-specific responses for BAL CD4 and CD8 T measured by ICS as in Fig. 1B at the indicated time points postimmunization. (B) The quality of the BAL CD4 and CD8 T-cell responses to 85A/B peptides (week 17) as shown for rAd5 in Fig 1E.

Kaimei Song, et al. Proc Natl Acad Sci U S A. 2010 December 21;107(51):22213-22218.
2.
Fig. 2.

Fig. 2. From: Genetic immunization in the lung induces potent local and systemic immune responses.

Humoral responses induced by aerosolized rAd5 immunization. (A and B) Induction of IgG (A) and IgA (B) to the vector inserts, SIV Gag-Pol (Left) and SIV Env (Right), were measured in serum. Data are shown as specific activity, i.e., micrograms of antigen-specific antibody of a given isotype per milligrams of total antibody of that isotype for each animal (n = 3 per group). The symbols filled with light blue represent one animal that had high preimmunization anti-gag-pol cross-reactivity. (C and D) rAd5 dose–response induction of HIV EnvA-specific IgG (C) and IgA (D) is shown in serum and three mucosal sites.

Kaimei Song, et al. Proc Natl Acad Sci U S A. 2010 December 21;107(51):22213-22218.
3.
Fig. 4.

Fig. 4. From: Genetic immunization in the lung induces potent local and systemic immune responses.

Aerosolized rAd5 provides protective immunity to live viral challenge. Three groups of ferrets (n = 15 each) were immunized using a sham rAd5 (by aerosol) or an rAd5-encoding influenza HA (Indonesia) by i.m. or aerosol. Eighteen days later, five animals in each group were euthanized to measure cellular responses; the remaining 10 animals were challenged with a lethal dose of H5N1 (Vietnam). (A) Protection in vaccinated groups was 100%. (B) Relative weight compared with day 0. (C) Viral loads measured from nasal swabs. (D) Induction of IFN-γ mRNA in BAL cells following restimulation with HA peptides ex vivo; each bar represents one animal measured in triplicate. (Error bar: +1 SD.) (E) Neutralization of the Indonesia H5N1 vaccine strain by sera from all 15 animals at the time of challenge. Note: a single animal in the aerosol group, indicated by the orange symbol, showed no neutralization, higher nasal viral loads, and significant weight loss but met no other criteria for euthanasia before the termination of the experiment.

Kaimei Song, et al. Proc Natl Acad Sci U S A. 2010 December 21;107(51):22213-22218.
4.
Fig. 1.

Fig. 1. From: Genetic immunization in the lung induces potent local and systemic immune responses.

Cellular immune responses induced by aerosolized rAd5 immunization. (A) After rAd5 immunization at week 0 and week 3, T-cell responses were measured by IFN-γ ELISpot to SIV Gag-Pol and Env peptides in PBMC. SFC, spot-forming cells. (B) Robust and persistent BAL CD4 and CD8 T-cell responses were measured by ICS. The responses to SIV Gag-Pol and Env peptides are summed. (C) A dose titration of rAd5 showed little effect on the total BAL T-cell magnitude when measured 8 wk after a single immunization. (D) The quality of the T-cell response induced by various doses of rAd5. The fraction of the response that is polyfunctional (3+) or solely IFN-γ is indicated by arrows. The quality also is summarized in pie charts depicting the polyfunctionality of the response: Antigen-specific cells secreting a single cytokine (1+) are depicted in light gray, cells secreting two cytokines (2+) are shown in dark gray, and cells secreting all three cytokines (3+) are shown in black. (E) Three animals were immunized with 1011 particles of rAd5 encoding SIV Env and Gag-Pol by aerosol (brown) or were immunized by aerosol and i.m. simultaneously (green); de novo responses to the SIV proteins are shown (Left). (Neutralizing titters in the serum following this first immunization are shown in Table 1.) These six animals then were immunized with 1010 particles of aerosolized rAd5 encoding HIV EnvA (Center); shown are the memory responses to SIV proteins (Center Left) and the de novo responses to HIV proteins (Center Right). For comparison, the de novo HIV responses in three different animals that received only aerosolized HIV Env rAd5 are shown (Right). (F) Ten animals were immunized with 5 × 109 particles of rAd5 encoding SIV Env. Seven of these animals were seronegative for Ad5 at the time of immunization (black), and three were seropositive from naturally acquired infection (purple).

Kaimei Song, et al. Proc Natl Acad Sci U S A. 2010 December 21;107(51):22213-22218.

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