Results: 2

1.
Fig. 2

Fig. 2. From: Effect of PCR extension temperature on high-throughput sequencing.

Coverage of sequence reads over DNA fragments with different AT contents obtained under different extension temperatures. (a) distribution of sequenced reads with different AT contents. Temperatures labeled with ‘r’ are plots from raw sequence reads; those with ‘m’ are plots from mapped reads. (b) mean fraction of coverage of 100-bp chromosomal segments with different AT contents; (c) ratios of fraction of coverage (60°C/70°C) at various AT contents; (d) plots of ratios of fraction of coverage (60°C/70°C) within 100-bp fragments having AT content < 80% (blue) and fragments with AT > 80% (red); (e) the same plots as those in b after excluding the fragments without any sequence read coverage; (f) depth of coverage of 100-bp genome fragments with different AT contents.

María José López-Barragán, et al. Mol Biochem Parasitol. ;176(1):64-67.
2.
Fig. 1

Fig. 1. From: Effect of PCR extension temperature on high-throughput sequencing.

Coverage of sequence reads at AT-rich and GC-rich regions amplified under different extension temperatures. Images of coverage plot from IGV genome browser (http://www.broadinstitute.org/igv/) displaying (a) a 530-bp AT-rich intergenic region on chromosome 6 (673,610– 674,140 bp) with good coverage when amplified at 60°C, with reduced coverage at 65°C, and with almost no coverage at 70°C, and (b) a 400-bp coding region on chromosome 6 (1,075,670–1,076,110 bp) with increased fold of coverage at two regions when amplified at 70°C. The AT contents of both the regions are indicated at the top of the figures.

María José López-Barragán, et al. Mol Biochem Parasitol. ;176(1):64-67.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk