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Results: 9

1.
FIGURE 2

FIGURE 2. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

EDC activation chemistry: preparing carboxyl groups for covalent attachment to exposed amine groups on antibodies.

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.
2.
FIGURE 4

FIGURE 4. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

Anti-IgG-HRP surface density on beads containing adsorbed antibodies, beads containing covalently bound antibodies, and beads containing PLL-crosslinked antibodies. (n = 3 for each type of bead).

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.
3.
FIGURE 6

FIGURE 6. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

TNF removal during 4-h recirculation using CADs packed with unmodified beads containing no antibody (◆), beads containing adsorbed anti-TNF (□), and beads containing adsorbed IgG (○). (n = 3 for each type of beads).

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.
4.
FIGURE 1

FIGURE 1. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

Bead modification chemistry: oxidation of the PSDVB portion of CytoSorb beads to incorporate carboxyl groups. Note that this schematic does not account for the presence of the PVP coating.

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.
5.
FIGURE 7

FIGURE 7. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

Comparison of the simultaneous removal of IL-6 (a), IL-10 (b), and TNF (c) using unmodified beads containing no antibodies (◆) and beads containing adsorbed anti-TNF antibodies (□). (n = 3 for both types of beads).

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.
6.
FIGURE 3

FIGURE 3. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

Experimental setup of cytokine capture experiments. The CAD is connected in line with a peristaltic pump and a reservoir of serum spiked with one or more cytokines.

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.
7.
FIGURE 8

FIGURE 8. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

Rate of anti-IgG-HRP leaching (◆) and overall amount lost (□) during 2-h PBS/BSA flush of beads containing adsorbed antibody. This flushing step was performed prior to the cytokine capture shown in Figure 9. (n = 3).

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.
8.
FIGURE 5

FIGURE 5. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

TNF removal during 4 h recirculation using CADs packed with unmodified beads containing no antibodies (◆), beads containing covalently bound anti-TNF (○), and beads containing adsorbed anti-TNF (□). (n = 3 for each type of beads).

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.
9.
FIGURE 9

FIGURE 9. From: Selective improvement of tumor necrosis factor capture in a cytokine hemoadsorption device using immobilized anti-tumor necrosis factor.

Comparison of TNF removal using beads containing adsorbed anti-TNF with (◆, solid line) and without (□, dashed line) 2-h preflush. The results of the flushing step performed on the beads used in these experiments can be seen in Figure 8. (n = 3 for both types of beads).

Morgan V. DiLeo, et al. J Biomed Mater Res B Appl Biomater. ;96(1):127-133.

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