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Results: 7

1.
FIGURE 7.

FIGURE 7. From: MicroRNA-210 Regulates Cancer Cell Proliferation through Targeting Fibroblast Growth Factor Receptor-like 1 (FGFRL1).

Effects of functional inhibition of endogenous miR-210. A, effect of inhibition of endogenous miR-210 on FGFRL1 expression levels was assessed by qRT-PCR and Western blot analyses using KYSE-590 cells. Cells were incubated for 48 h after transfection with anti-ncRNA or anti-miR-210. The values are shown relative to the value obtained with anti-ncRNA (n = 3; **, p < 0.05 versus anti-ncRNA). B, cell viability in A was evaluated by the WST-1 assay. The values are shown relative to the value obtained with anti-ncRNA (n = 3; **, p < 0.05 versus anti-ncRNA).

Soken Tsuchiya, et al. J Biol Chem. 2011 January 7;286(1):420-428.
2.
FIGURE 5.

FIGURE 5. From: MicroRNA-210 Regulates Cancer Cell Proliferation through Targeting Fibroblast Growth Factor Receptor-like 1 (FGFRL1).

Identification of miR-210 target sites in FGFRL1 3′-UTR. A, schematic diagram of potential miR-210-target sites in FGFRL1 3′-UTR. B–G, cells were transfected with either ncRNA or miR-210 at 24 h after transfection with the pGL3 luciferase expression vector containing 3′-UTR of each gene or candidate miR-210 target site in FGFRL1 3′-UTR. After 14 h, reporter luciferase activity was evaluated. The values are shown relative to the value obtained with ncRNA (n = 3; *, p < 0.01).

Soken Tsuchiya, et al. J Biol Chem. 2011 January 7;286(1):420-428.
3.
FIGURE 1.

FIGURE 1. From: MicroRNA-210 Regulates Cancer Cell Proliferation through Targeting Fibroblast Growth Factor Receptor-like 1 (FGFRL1).

Down-regulation of miR-210 expression in ESCC. A and B, expression levels of miR-210 and miR-31 in NEST and ESCC assessed by qRT-PCR. The values are shown relative to the value obtained for NEST (n = 82; *, p < 0.01). C, clinicopathologic characteristics of 82 ESCCs divided into two groups (n = 41) on the basis of miR-210 expression levels. D, expression levels of miR-210 in the ESCCs and the corresponding NESTs in each histological type were compared by qRT-PCR. The values are shown relative to the value obtained for NEST in the well differentiated group (**, p < 0.05; *, p < 0.01). E, levels of miR-210 in HE3, HEEpiC, and KYSE cell lines analyzed by qRT-PCR. The values are shown relative to the value obtained for HE3 (n = 3; *, p < 0.05 versus HE3; #, p < 0.05 versus HEEpiC). Error bars, S.E.

Soken Tsuchiya, et al. J Biol Chem. 2011 January 7;286(1):420-428.
4.
FIGURE 2.

FIGURE 2. From: MicroRNA-210 Regulates Cancer Cell Proliferation through Targeting Fibroblast Growth Factor Receptor-like 1 (FGFRL1).

Functions of miR-210 in KYSE-170 cells. A, effect of miR-210 on cell proliferation was investigated by the WST-1 assay. Cells were incubated for 48 h after transfection with either ncRNA or miR-210, and viability was evaluated. The values are shown relative to the value obtained with ncRNA. B, expression levels of miR-210 in cells at 48 h after transfection with either ncRNA or miR-210 were investigated by qRT-PCR. The values are shown relative to the value obtained with ncRNA. C, 48 h after transfection, cell proliferation was evaluated by BrdU incorporation. The values are shown relative to the value obtained with ncRNA. D, effects of miR-210 on the cell cycle were investigated. The PI-stained DNA content of the cells was evaluated using a FACScan flow cytometer at 48 h after transfection. E, at 48 h after transfection, cells were stained with FITC-conjugated annexin V, and PI and cell death was evaluated using a FACScan flow cytometer. n = 3; **, p < 0.05; *, p < 0.01 versus ncRNA.

Soken Tsuchiya, et al. J Biol Chem. 2011 January 7;286(1):420-428.
5.
FIGURE 3.

FIGURE 3. From: MicroRNA-210 Regulates Cancer Cell Proliferation through Targeting Fibroblast Growth Factor Receptor-like 1 (FGFRL1).

Identification of candidate target genes degraded by miR-210 in KYSE-170 cells. A, Venn diagram showing overlapping sets of genes whose expression was decreased >5-fold by transfection of miR-210 and computationally predicted target genes of miR-210. B, list of four potential miR-210 target genes with a signal value of more than 50 in ncRNA-transfected cells upon microarray analysis. C–F, expression levels of the four potential miR-210 target mRNAs assessed by qRT-PCR. The values are shown relative to the value obtained with ncRNA (n = 3; *, p < 0.01). G, Western blot analyses of FGFRL1, NDUFA4, GPR177, LRP5L, and β-actin proteins.

Soken Tsuchiya, et al. J Biol Chem. 2011 January 7;286(1):420-428.
6.
FIGURE 4.

FIGURE 4. From: MicroRNA-210 Regulates Cancer Cell Proliferation through Targeting Fibroblast Growth Factor Receptor-like 1 (FGFRL1).

Identification of candidate target genes of miR-210 in ESCC. A–D, FGFRL1, NDUFA4, GPR177, and LRP5L expression levels in ESCCs divided into two groups on the basis of miR-210 expression levels were assessed by qRT-PCR. The values are shown relative to the value obtained for the miR-210-low group (n = 41; **, p < 0.05; *, p < 0.01). E, clinicopathologic characteristics of 82 ESCCs divided into two groups (n = 41) on the basis of FGFRL1 expression levels. F, plot of log10FGFRL1 relative expression intensity against log10miR-210 relative expression intensity. The line represents an approximated curve. The correlation coefficient (r) and the p value indicate the statistical significance of the negative correlation between the x and y variables. G, immunohistochemistry for FGFRL1 on ESCC and NEST. These sections were stained by anti-FGFRL1 antibody and by hematoxylin and eosin (H&E).

Soken Tsuchiya, et al. J Biol Chem. 2011 January 7;286(1):420-428.
7.
FIGURE 6.

FIGURE 6. From: MicroRNA-210 Regulates Cancer Cell Proliferation through Targeting Fibroblast Growth Factor Receptor-like 1 (FGFRL1).

Functions of FGFRL1 in KYSE-170 cells. A, effect of FGFRL1 on cell proliferation was investigated by the WST-1 assay. Cells were incubated for 48 h after transfection with ncsiRNA, FGFRL1 siRNA1 or 2, and viability was evaluated. The values are shown relative to the value obtained with ncsiRNA (n = 3; *, p < 0.01 versus ncsiRNA). B, FGFRL1 expression levels in A were assessed by qRT-PCR. The values are shown relative to the value obtained with ncsiRNA (n = 3; *, p < 0.01 versus ncsiRNA). C, effects of FGFRL1 on the cell cycle were investigated. The PI-stained DNA content of the cells was evaluated using a FACScan flow cytometer at 48 h after transfection (n = 3; **, p < 0.05; *, p < 0.01 versus ncsiRNA). D, at 48 h after transfection, cells were stained with FITC-conjugated annexin V and PI, and cell death was evaluated using a FACScan flow cytometer (n = 3). E, cells were transfected with either ncRNA or miR-210 at 24 h after transfection with the FGFRL1 expression vector or mock. After 48 h, viability was evaluated. The values are shown relative to the value obtained with ncRNA and mock transfection (n = 3; *, p < 0.01). F, Western blot analyses of FGFRL1 and β-actin proteins in E are shown.

Soken Tsuchiya, et al. J Biol Chem. 2011 January 7;286(1):420-428.

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