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Figure 2

Figure 2. Two dimensional representation of MALDI-IM-MS data. From: Metabolic Profiling of Escherichia coli by Ion Mobility-Mass Spectrometry with MALDI Ion Source.

A1-background ions (DHB matrix + blank E. coli extrat) , A2-Zoomed in the m/z range 650-850 Da, B1- sample ions (DHB matrix + E. coli extract), and B2- Zoomed in the m/z range 650–850 Da. On the x-axis is shown the mass-to-charge ratio of the ions in Da and the y-axis represents the drift times of ions in μs.

Prabha Dwivedi, et al. J Mass Spectrom. ;45(12):1383-1393.
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Figure 5

Figure 5. ESI-IM-MS contour diagram of intracellular E. coli metabolome. From: Metabolic Profiling of Escherichia coli by Ion Mobility-Mass Spectrometry with MALDI Ion Source.

Drift time of an ion in microseconds is plotted against the mass to charge ratio of the ion. The spectrum on the top covers m/z range of 60-600 Da. The bottom spectrum is a close up view in the m/z range of 150–250 Da.

Prabha Dwivedi, et al. J Mass Spectrom. ;45(12):1383-1393.
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Figure 7

Figure 7. Illustration of the two dimensional space (shaded area in the X-Y quadrant) occupied by ions detected from intracellular E. coli extract by IM-MS with MALDI (7a) and ESI (7b) as the ionization source. From: Metabolic Profiling of Escherichia coli by Ion Mobility-Mass Spectrometry with MALDI Ion Source.

The theoretical trend line (dotted line) is the centre line of the shaded area along which a constant drift time deviation should be observed. Trend line for the experimental data is depicted as bold line. The length of the 2D space is defined by the m/z range and double sided arrow defines the drift time spread in the given m/z range.

Prabha Dwivedi, et al. J Mass Spectrom. ;45(12):1383-1393.

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