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Results: 5

1.
F <span style="font-variant: small-caps" class="small-caps">igure</span>  2.—

F igure 2.—. From: Functional Redundancy of Paralogs of an Anaphase Promoting Complex/Cyclosome Subunit in Caenorhabditis elegans Meiosis.

such-1(av9gf) animals are fertile and healthy. Wild-type and such-1(av9gf) animals were grown at 16° and 24°. Brood size (A) and percentage of embryonic hatching (B) were evaluated and found to be not significantly different. Statistics, Student's T-test; error bars indicate standard deviation.

Kathryn K. Stein, et al. Genetics. 2010 December;186(4):1285-1293.
2.
F <span style="font-variant: small-caps" class="small-caps">igure</span>  1.—

F igure 1.—. From: Functional Redundancy of Paralogs of an Anaphase Promoting Complex/Cyclosome Subunit in Caenorhabditis elegans Meiosis.

such-1 RNAi eliminates the suppression of mat-3(or180ts) by such-1(av9). Wild-type, mat-3(or180ts), and mat-3(or180ts) such-1(av9) animals were grown at 24° on smd-1 control or such-1(exons 6-8) RNAi and embryonic hatching was assessed. 1C indicates the presence of arrested one-cell embryos. Statistics, Student's T-test; asterisk indicates a P-value <0.001. Error bars indicate standard deviation.

Kathryn K. Stein, et al. Genetics. 2010 December;186(4):1285-1293.
3.
F <span style="font-variant: small-caps" class="small-caps">igure</span>  3.—

F igure 3.—. From: Functional Redundancy of Paralogs of an Anaphase Promoting Complex/Cyclosome Subunit in Caenorhabditis elegans Meiosis.

such-1(av9gf) does not genetically interact with the spindle checkpoint mutants mdf-2(av14) or fzy-1(av15gf). Double mutants were made with dpy-18(e364) such-1(av9gf) and two spindle checkpoint mutants, unc-17(e245) mdf-2(av14) (A and B) and fzy-1(av15gf) unc-4(e120) (C and D). Brood size per animal (A and C) and embryonic hatching (B and D) are not significantly different between the control and experimental strain. Statistics, Student's T-test; error bars indicate standard deviation.

Kathryn K. Stein, et al. Genetics. 2010 December;186(4):1285-1293.
4.
F <span style="font-variant: small-caps" class="small-caps">igure</span>  4.—

F igure 4.—. From: Functional Redundancy of Paralogs of an Anaphase Promoting Complex/Cyclosome Subunit in Caenorhabditis elegans Meiosis.

such-1 and gfi-3 transcriptional fusions are expressed predominantly in the adult germline and embryo. such-1∷mCherry∷H2B∷such-1 and gfi-3∷mCherry∷H2B∷gfi-3 stable transgenes were imaged by fluorescence microscopy (A–H) and DIC (B, C, F, and G). such-1 (A) and gfi-3 (E) adult hermaphrodites exhibit expression in the germline extending from the distal tip (inset) to the −1 oocyte (arrowhead, A and E). Sperm expression is observed in the spermathecae (arrow, A and E). Somatic gut nuclei (asterisk, E) also express gfi-3. such-1 is expressed in meiotic (arrow, B) and mitotic embryos (C). gfi-3 is expressed at all embryonic stages, including meiosis (arrow, F) and mitosis (G). Male germlines also express the such-1 (D) and gfi-3 (H) transgenes; mature sperm are indicated with an arrow. Bars, 10 μm.

Kathryn K. Stein, et al. Genetics. 2010 December;186(4):1285-1293.
5.
F <span style="font-variant: small-caps" class="small-caps">igure</span>  5.—

F igure 5.—. From: Functional Redundancy of Paralogs of an Anaphase Promoting Complex/Cyclosome Subunit in Caenorhabditis elegans Meiosis.

such-1 and gfi-3 act redundantly in meiosis. (A) Schematic of such-1 and gfi-3 gene structure and position of genomic fragments used in RNAi constructs (solid bars below gene structure). The scale bar to the bottom right of each gene structure indicates 100 base pairs (bp). Total genomic length is to the right of the gene structure. (B) Percentage of embryonic hatching of wild-type and such-1(h1960ts) animals grown on several different RNAi conditions at 25°. N > 750 for each sample. (C) Percentage of dead embryos exhibiting a one-cell–arrest phenotype at 25°. N > 435 for each sample. Statistics, Student's T-test; asterisk indicates a significant difference between sample and smd-1 control, with a P-value <0.05. Error bars indicate standard deviation.

Kathryn K. Stein, et al. Genetics. 2010 December;186(4):1285-1293.

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