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1.
Figure 6

Figure 6. From: Alcohol Inhibits Smooth Muscle Cell Proliferation via Regulation of the Notch Signaling Pathway.

A and B, Quantitative RT-PCR analysis of Notch 1 and Notch 3 mRNA (A) and HRT-1 mRNA (B) isolated from whole carotid arteries of control or alcohol (EtOH)–fed C57Bl/6J mice, 2 weeks after partial ligation or sham operation. Data are normalized to GAPDH (n=5). *P≤0.05 vs control ligated.

David Morrow, et al. Arterioscler Thromb Vasc Biol. ;30(12):2597-2603.
2.
Figure 4

Figure 4. From: Alcohol Inhibits Smooth Muscle Cell Proliferation via Regulation of the Notch Signaling Pathway.

The effect of daily moderate alcohol feeding on vessel remodeling after carotid artery ligation. Carotid artery vessel compartment volumes (evaluated over a 1-mm carotid length) for control and EtOH-fed, sham-operated, and ligated groups. Data are given as the mean±SEM, 4 sections analyzed per animal (n=3 animals [sham], and n=6 animals [ligated]). *P≤0.05 vs control ligated.

David Morrow, et al. Arterioscler Thromb Vasc Biol. ;30(12):2597-2603.
3.
Figure 5

Figure 5. From: Alcohol Inhibits Smooth Muscle Cell Proliferation via Regulation of the Notch Signaling Pathway.

Immunohistochemical staining for PCNA and α-actin in representative sections of carotid arteries of C57BL/6J mice, 14 days after sham operation (Sham) or partial ligation (Ligated) in control or alcohol-fed animals. Verhoeff–van Gieson–stained sections are also shown. The arrows denote internal and external elastic laminae (original magnification ×10). The scale bar indicates 100 μm.

David Morrow, et al. Arterioscler Thromb Vasc Biol. ;30(12):2597-2603.
4.
Figure 1

Figure 1. From: Alcohol Inhibits Smooth Muscle Cell Proliferation via Regulation of the Notch Signaling Pathway.

EtOH inhibits SMC proliferation. A, HCASMCs were treated with or without EtOH (25 mmol/L). Cell counts of parallel triplicate wells were performed daily. Representative growth curves are shown. The experiment was repeated with similar results. B, Representative Western blot of PCNA protein expression in HCASMCs treated with or without EtOH (25 mmol/L for 24 hours). C, Flow cytometric analysis of stained HCASMCs (Annexin V/7-AAD). Percentages of apoptotic cells are shown in the top left quadrant for each condition. PE indicates phycoerythrin.

David Morrow, et al. Arterioscler Thromb Vasc Biol. ;30(12):2597-2603.
5.
Figure 3

Figure 3. From: Alcohol Inhibits Smooth Muscle Cell Proliferation via Regulation of the Notch Signaling Pathway.

Notch 1 IC or HRT-1 overex-pression blocks inhibition of SMC proliferation by EtOH. A and B, SMCs were transfected without (mock) or with constitutively active Notch 1 IC (A) or hHRT-1 (B) and treated with EtOH for 24 hours; PCNA protein levels were measured by Western blot as an index of proliferation. Blots were reprobed for β-actin or GAPDH as a loading control. A, *P<0.05 vs mock control (0-mmol/L EtOH). A representative Western blot is shown, with cumulative data (n=3). B, Top, A representative Western blot of 3 is shown; bottom, growth curves for control and EtOH-treated cells, with or without HRT-1 overexpression.

David Morrow, et al. Arterioscler Thromb Vasc Biol. ;30(12):2597-2603.
6.
Figure 2

Figure 2. From: Alcohol Inhibits Smooth Muscle Cell Proliferation via Regulation of the Notch Signaling Pathway.

EtOH inhibits Notch signaling in HCASMCs. A and B, Quantitative RT-PCR analysis of Notch 1 and Notch 3 receptors (A) and Notch target gene HRT-1 and HRT-2 mRNA (B) after EtOH (25 mmol/L) treatment for 24 hours. Data are normalized to GAPDH and represent the mean±SEM (n=4). C, Representative Western blots, together with cumulative data showing a decrease in Notch 1 IC but no effect on Notch 3 IC with EtOH (25 mmol/L) treatment. (Blots were reprobed for β-actin as a loading control.) Data are given as the mean±SEM (n=4). D, CBF-1/RBP-JK–dependent promoter activity, evaluated by luciferase assay and normalized to β-galactosidase activity, after EtOH treatment for 24 hours. Data are given as the mean±SEM (n=3). *P≤0.05 vs control.

David Morrow, et al. Arterioscler Thromb Vasc Biol. ;30(12):2597-2603.

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