Results: 5

1.
Figure 5

Figure 5. From: Semiquantitative histopathology and 3D magnetic resonance microscopy as collaborative platforms for tissue identification and comparison within teratomas derived from pedigreed primate embryonic stem cells.

Histological section (left) co-registered with 2D MRM slice (right). Tissues and structures that are clearly differentiated in both histology and MRM are outlined in color. These outlines represent adipose tissue (cyan), cartilage (orange), cystic regions (red, yellow), toothpicks inserted as fiduciary markers (green), and epidermis from skin (blue). Some tissue patches that appear in histology do not appear in MRM because they either do not generate MR contrast, or their contrast was diminished due to MR image slice thickness -- because the histological section is 5 μm thick, the 180 μm-thick MR image slice represents the sum of 36 histological slices. Finally, some of the tissue that is present in the MR image slice is missing from the histological section, which is an artifact introduced during the sectioning process.

Carlos A. Castro, et al. Stem Cell Res. ;5(3):201-211.
2.
Figure 4

Figure 4. From: Semiquantitative histopathology and 3D magnetic resonance microscopy as collaborative platforms for tissue identification and comparison within teratomas derived from pedigreed primate embryonic stem cells.

Volumetric teratoma data compiled from 3D MRM segmentation of three teratomas (rows A, B, C). From left to right: Gross Volume represents teratoma volume including cysts and mouse tissues; Solid Tissue (gray) represents teratoma volume after removing cysts and mouse tissues; Cyst (yellow) represents percentage of Gross Volume that is liquid-filled cyst; and Low Intensity Tissue represents the percentage of Solid Tissue that exhibits the lowest 36 ± 7% of MR image intensities after cross-teratoma normalization. The 36% intensity threshold was chosen since tissues below it would tend to have structure and texture (e.g., patches, lines, and punctate spots). The three red cylinders represent toothpicks that were inserted through the volume as fiducial markers to enhance MRM-histological registration and correlation.

Carlos A. Castro, et al. Stem Cell Res. ;5(3):201-211.
3.
Figure 1

Figure 1. From: Semiquantitative histopathology and 3D magnetic resonance microscopy as collaborative platforms for tissue identification and comparison within teratomas derived from pedigreed primate embryonic stem cells.

Pipeline for MRM and histopathological analysis of teratomas derived from pedigreed nhpESCs: After isolation (A) and several days of fixation in 10% buffered formalin, wooden fiduciary markers are inserted and the teratoma is placed in a 50 ml conical tube with Fluorinert for MRM imaging (B, F). Representative MR images are shown in panels C, D, E. G-I show the path for histopathological analysis from cassette to hematoxylin and eosin stained slide. In this case the teratoma required 12 cassettes for submission of the entire tumor. J represents the alignment of one of the whole slide scanned images to its corresponding MR image. K demonstrates the 3D rendering of the entire teratoma with fiduciary markers in place.

Carlos A. Castro, et al. Stem Cell Res. ;5(3):201-211.
4.
Figure 3

Figure 3. From: Semiquantitative histopathology and 3D magnetic resonance microscopy as collaborative platforms for tissue identification and comparison within teratomas derived from pedigreed primate embryonic stem cells.

Tumor derived from 106-A1 and A2 lines. A-C are hematoxylin and eosin stained images of the tumor. In panel A, many areas showed glandular profiles composed of cells with basally oriented nuclei and abundant amphophilic cytoplasm (200X). Focal areas showed cells with a more clear or vacuolated cytoplasm (B) (100X). C shows higher magnification view of glandular profile. Scattered apoptotic debris is present (400X). A selected immunohistochemistry profile is shown in panels D-H. Tumor cells were positive for both cytokeratin and vimentin (D, E respectively, 200X). Smooth muscle actin and all muscle actin stains highlighted scattered spindle cells interspersed between glandular profiles. Focal light staining of some glandular cells was also seen (F-smooth muscle actin; 200X, G-all muscle actin; 400X). S100 stain (H) showed a rare positive interglandular cell (arrows) (S100; 400X).

Carlos A. Castro, et al. Stem Cell Res. ;5(3):201-211.
5.
Figure 2

Figure 2. From: Semiquantitative histopathology and 3D magnetic resonance microscopy as collaborative platforms for tissue identification and comparison within teratomas derived from pedigreed primate embryonic stem cells.

Collage of images of hematoxylin and eosin stained tissue sections representing the range of tissues present in teratomas from pedigreed nhpESC lines. A-E represent ectodermal derivatives, F-O-mesodermal derivatives, P-T-endodermal derivatives, and U-Y-interesting isolated findings. A-Neuroepithelium showing stratified epithelium arranged in ribbons and rosettes (40X). B-Immature neuroglia focus composed of neuroblastic cells (200X). A few pigmented cells within rosettes are present and may represent early retinal anlagen (arrows). C-Mature neuroglia containing both neurons and glial cells. A remnant of a testicular tubule is present in the upper right (200X). D-Mature ganglion (100X). E-Well-developed skin with adnexal structures and hair follicles extending deep into the surrounding adipose tissue (20X). F-Mature smooth muscle with a developing myenteric plexus containing immature ganglion cells (arrow) (200X). G-Immature smooth muscle surrounding an immature gland (100X). H-Immature skeletal muscle showing multinucleated myotubes and rhabdomyoblasts within cellular stroma (400X). I-Mature skeletal muscle composed of long striated myofibers (400X). J-Mature skeletal muscle comprising a large area of tissue in the 3106 line (100X). K-Focus of immature adipose tissue. Note the variably sized individual adipocytes and lipoblasts with centrally located nuclei (arrows) (200X). L-Mature adipose tissue showing a lobule adjacent to bone (right) (100X). Note the generally uniform size of adipocytes. M-Cellular immature mesenchyme seen beneath developing epithelium (top right)(200X). The cells have spindle-shape nuclei and scattered mitoses. N-Fibromuscular stroma (200X). O-Mature bone without bone marrow (100X). P-Immature gland surrounded by immature smooth muscle. Some glandular cells show apical clear cytoplasm (100X). Q-Collection of immature ectactic glands some with a staghorn appearance surrounded by immature smooth muscle/fibromuscular stroma. This collection resembles immature prostate tissue (100X). R-Isolated immature gland showing a few early epithelial buddings (100X). S-Immature gland composed of stratified cuboidal epithelium with hint of a basal layer. Note adjacent cartilage (100X). T-Focus of immature tissue resembling yolk sac. Many of the cells have sub- and supranuclear cytoplasmic clearing (piano keys) (100X). U-Pigmented cells lining a glandular space (400X). V-Glands resembling salivary gland near an epithelial structure (duct) (lower right)(200X). W-Intimately associated nerve and muscle resembling a rare pathological entity seen in young children; neuromuscular hamartoma (100X). X, Y-Developing tooth seen in 3106. X shows immature tooth organ with central pulp (P) surrounded by dentin layer (D) and ameloblastic epithelium (Am) (100X). Y-Tooth bell from same teratoma (20X).

Carlos A. Castro, et al. Stem Cell Res. ;5(3):201-211.

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