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Results: 5

1.
Figure 3

Figure 3. Multiple sequence alignment. From: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface.

The amino acid sequences were aligned for HRPE773 (also known as human CSP-1, GenBank AAQ89380.1) and rat CSP-1 (Accession no. NP598306.1) sourced from from NCBInr. Amino acid identities across both species are indicated by an asterisk. Individual peptides identified through MS analysis are mapped to the HRPE773 protein sequence (amino acids in bold italics) and provide 51% sequence coverage, validating the identification of the protein as CSP-1.

Kiran S. Ambatipudi, et al. J Proteome Res. ;9(12):6605-6614.
2.
Figure 1

Figure 1. Human salivary proteins eluted from the surface of S. mutans. From: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface.

Venn diagram showing the human salivary proteins differentially bound to the surface of S. mutans, as grouped by the number of overlapping and non-overlapping proteins in the different affinity pools: 0.85% salt (weakly bound, pool A), 1 M salt (moderately bound, pool B) and 2M urea (tightly bound, pool C) solutions.

Kiran S. Ambatipudi, et al. J Proteome Res. ;9(12):6605-6614.
3.
Figure 5

Figure 5. Recombinant human CSP-1 protein (rCSP-1) enhances bacterial adherence to experimental salivary pellicle (sHA) and to glucans synthesized in the pellicle (gsHA) formed on hydroxyapatite surface. From: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface.

Modulation of bacterial adherence to sHA (panel A) and gsHA (panel B) by S. mutans exposed to rCSP-1 (in PBS or in clarified human whole saliva, left and right, respectively). The data shown are mean values ± standard deviations (n=6). Values marked with an asterisk are significantly different from each other (P<0.05, ANOVA, comparison for all pairs using Tukey’s test).

Kiran S. Ambatipudi, et al. J Proteome Res. ;9(12):6605-6614.
4.
Figure 4

Figure 4. Structure, purification and validation of the recombinant human CSP-1 protein (rCSP-1). From: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface.

Human CSP-1 was cloned and the recombinant protein expressed in S2 cells. (A) The rCSP-1 protein contains an N-terminal secretory signal peptide (SP; black box), FLAG epitope tags (grey box), six histidine residues (dotted black box), metal chelate tag (empty oval), S-peptide tag (striped oval), 3c protease cleavage site (dotted box) and soluble region (white box). Note that the CSP-1 portion of the recombinant protein is not to scale so that the N-terminal region can be more easily visualized. (B) SDS-PAGE (4–12%) analysis of rCSP-1 stained with Simply Blue. Lane 1, metal chelate affinity chromatography of rCSP-1; lane 2, molecular weight markers; lane 3, rCSP-1 re-purified using con A lectin affinity chromatography.

Kiran S. Ambatipudi, et al. J Proteome Res. ;9(12):6605-6614.
5.
Figure 2

Figure 2. Comparison of overlapping human salivary proteins eluted from the surface of S. mutans. From: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface.

A comparison of the fifteen human salivary proteins bound to the surface of S. mutans present in all three affinity pools was performed based on their spectral counts: 0.85% salt (weakly bound, pool A), 1 M salt (moderately bound, pool B) and 2M urea (strongly bound, pool C) solutions. (A) Comparison of the nine most abundant proteins (spectral counts >160) identified in pools A, B and C. (B) Comparison of the six least abundant proteins (spectral counts <160) detected in pools A, B and C. CSP-1 = common salivary protein-1; Salivary agglutinin (gp340) = deleted in malignant brain tumor 1; Polymeric-Ig precursor = Polymeric-immunoglobulin precursor; P-I protein precursor = Prolactin-inducible protein precursor; Basic salivary PRP 1 precursor = Basic salivary proline rich protein 1 precurser; CA VI precursor = Carbonic anhydrase VI precursor; SPLNEC = short palate, lung & nasal epithelium carcinoma.

Kiran S. Ambatipudi, et al. J Proteome Res. ;9(12):6605-6614.

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