We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 5

1.
FIG. 1.

FIG. 1. From: Multiple Antigen Peptide Vaccines against Plasmodium falciparum Malaria .

Amino acid sequences of the MAP peptides and their physical locations on P. falciparum antigens. (A) MAP-1 vaccine. (B) MAP-2 vaccine. (C) MAP-3 vaccine.

Babita Mahajan, et al. Infect Immun. 2010 November;78(11):4613-4624.
2.
FIG. 4.

FIG. 4. From: Multiple Antigen Peptide Vaccines against Plasmodium falciparum Malaria .

(A) ELISPOT IFN-γ and IL-4 responses in HLA-A2, C57BL/6, and BALB/c mice immunized by three subcutaneous injections of MAP-1 vaccine in Montanide ISA 51. Freshly isolated spleen cells were pooled from five mice per group and cultured in the presence of MAP-1 or individual peptides (10 μg/ml). Data are presented as mean numbers of antigen-specific IFN-γ/IL-4 SFC per million spleen cells, and error bars reflect the standard deviation of triplicate samples. (B) ELISPOT IFN-γ and IL-4 responses in C57BL/6 and BALB/c mice immunized by three subcutaneous injections of MAP-2 vaccine in Montanide ISA 51. Freshly isolated spleen cells from five mice per group were pooled and cultured in the presence of MAP-2 or individual peptides (10 μg/ml). Data are presented as mean numbers of antigen-specific IFN-γ/IL-4 SFC per million spleen cells, and error bars reflect the standard deviations of triplicate samples.

Babita Mahajan, et al. Infect Immun. 2010 November;78(11):4613-4624.
3.
FIG. 3.

FIG. 3. From: Multiple Antigen Peptide Vaccines against Plasmodium falciparum Malaria .

IIF IgG reactivity against P. falciparum liver stage parasites in C57BL/6, A/J, BALB/c, and CD1 strains of mice that received three subcutaneous injections of MAP vaccines in Montanide ISA 51. The reactivity for liver stage parasites was tested against (A) polyclonal mouse anti-LSA-1 antibody (positive control), (B) MAP-2-immunized BALB/c mouse sera, (C) C57BL/6-immunized mouse sera, (D) CD1-immunized mouse sera, and (E) normal mouse serum. The arrow points to the developing P. falciparum parasites. The hepatocyte nucleus is stained blue with DAPI.

Babita Mahajan, et al. Infect Immun. 2010 November;78(11):4613-4624.
4.
FIG. 2.

FIG. 2. From: Multiple Antigen Peptide Vaccines against Plasmodium falciparum Malaria .

ELISA IgG responses in HLA-A2, C57BL/6, A/J, BALB/c, and CD1 strains of mice immunized by three subcutaneous injections of MAP vaccines in Montanide ISA 51 or injected with Montanide ISA 51 alone. ELISA IgG titers were determined in pooled sera (n = 6) from each immunization group. (A) Sera from MAP-1-immunized mice tested against MAP-1 or rPfCSP as a coating antigen. (B) Sera from MAP-2-immunized mice tested against MAP-2 or individual peptides. (C) Sera from MAP-3-immunized mice tested against MAP-3 or individual peptides. ELISA IgG titers are calculated values determined as interpolated titers at an optical density (OD) of 0.5. The bars represent mean ELISA IgG titers, and the error bars reflect the standard deviations of triplicate samples.

Babita Mahajan, et al. Infect Immun. 2010 November;78(11):4613-4624.
5.
FIG. 5.

FIG. 5. From: Multiple Antigen Peptide Vaccines against Plasmodium falciparum Malaria .

Merozoite growth/invasion-inhibitory activity of anti-MAP-3 sera against the P. falciparum 3D7, FVO, and Camp strains of asexual blood stage parasites. Synchronized ring stage parasites at 0.2% parasitemia were mixed with anti-MAP-3 sera (1:10 dilution) from the C57BL/6, A/J, BALB/c, and CD1 strains of mice in 48-well plates in suspension state. The cultures were harvested after 48 h, and parasitemia was evaluated by enumerating parasites stained with Hoechst dye 33342 and analyzing the fluorescence signal of approximately 40,000 erythrocytes by flow cytometry. Percent inhibition was calculated from the mean parasitemia of triplicate test and control wells as 100 × [(control − test/control)].

Babita Mahajan, et al. Infect Immun. 2010 November;78(11):4613-4624.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk