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1.
FIG. 4.

FIG. 4. From: Interaction of Candida albicans Cell Wall Als3 Protein with Streptococcus gordonii SspB Adhesin Promotes Development of Mixed-Species Communities .

Coaggregation of L. lactis expressing SspB with C. albicans hyphae. (B to I) FITC-labeled L. lactis MG1363 (Ll control) or L. lactis MG1363(pUB1000-sspB) (LlSspB) was incubated in suspension with hypha-forming cells of C. albicans NGY152 (CaWT) or the 1843 als3Δ/als3Δ mutant (CaΔALS3). Aggregates were visualized by light or fluorescence microscopy. (A) Percentages of hyphae with attached bacteria were calculated on the basis of bacterial binding levels, as described in the legend to Fig. 2. The data are means ± standard errors (SE) from 4 independent experiments (*, P < 0.05).

Richard J. Silverman, et al. Infect Immun. 2010 November;78(11):4644-4652.
2.
FIG. 3.

FIG. 3. From: Interaction of Candida albicans Cell Wall Als3 Protein with Streptococcus gordonii SspB Adhesin Promotes Development of Mixed-Species Communities .

Expression of heterologous proteins in L. lactis or S. cerevisiae. Lactococcal cells were incubated with antibodies to SspB, and antibody reactivity was detected with FITC-labeled anti-rabbit secondary antibody. (A and C) Light microscopy. (B and D) Fluorescence microscopy. (A and B) L. lactis MG1363 (control) cells nonreactive with antibody. (C and D) L. lactis expressing SspB. (E) Western blots of lyticase-extracted fungal cell wall proteins reacted with monoclonal antibody to Als3p and antibody binding detected with HRP-linked anti-mouse secondary antibody. Lane 1, S. cerevisiae wild type; lane 2, S. cerevisiae expressing Als3p; lane 3, C. albicans NGY152 wild type; lane 4, C. albicans als3Δ/als3Δ.

Richard J. Silverman, et al. Infect Immun. 2010 November;78(11):4644-4652.
3.
FIG. 5.

FIG. 5. From: Interaction of Candida albicans Cell Wall Als3 Protein with Streptococcus gordonii SspB Adhesin Promotes Development of Mixed-Species Communities .

Coaggregation between bacterial strains and S. cerevisiae expressing Als3p. FITC-labeled streptococci or lactococci were incubated with S. cerevisiae S150-2B parent cells or with cells constitutively expressing Als3p. Coaggregates were visualized by light or fluorescence microscopy. (A and B) S. cerevisiae S150-2B control cells mixed with L. lactis expressing SspB. (C and D) S. cerevisiae S150-2B expressing Als3p mixed with L. lactis expressing SspB, showing coaggregation. The arrows indicate yeast cells with or without adhered bacteria. (E) Percentages of S. cerevisiae parent (WT) or Als3p-expressing cells interacting with S. gordonii DL1 (Sg WT) or S. gordonii Δ(sspA sspB) [Sg Δ(sspAB)]. (F) Percentages of S. cerevisiae parent (WT) or Als3p-expressing cells interacting with L. lactis MG1363 control cells (Ll control) or L. lactis MG1363 expressing SspB (Ll SspB). The data are means and SD from 2 independent experiments (*, P < 0.05).

Richard J. Silverman, et al. Infect Immun. 2010 November;78(11):4644-4652.
4.
FIG. 1.

FIG. 1. From: Interaction of Candida albicans Cell Wall Als3 Protein with Streptococcus gordonii SspB Adhesin Promotes Development of Mixed-Species Communities .

Als3p in mono- or dual-species biofilms. (A) C. albicans wild type or C. albicans als3Δ/als3Δ biofilms that formed on saliva-coated glass at 1 h, 3 h, or 6 h stained with CV and visualized by microscopy. The images are representative of experiments performed in triplicate. Scale bars, 40 μm. (B) C. albicans wild type or C. albicans als3Δ/als3Δ biofilms that formed on S. gordonii DL1 cells at 1 h, 3 h, or 6 h stained with crystal violet and visualized by microscopy. The images are representative of experiments performed in triplicate. Scale bars, 25 μm. (C) Biomasses of biofilms of C. albicans WT or C. albicans ΔALS3 assessed by CV release. (D) Cell densities of planktonic-phase samples from developing C. albicans biofilms. The data are means and standard deviations (SD) from experiments performed in triplicate (*, P < 0.05).

Richard J. Silverman, et al. Infect Immun. 2010 November;78(11):4644-4652.
5.
FIG. 2.

FIG. 2. From: Interaction of Candida albicans Cell Wall Als3 Protein with Streptococcus gordonii SspB Adhesin Promotes Development of Mixed-Species Communities .

Coaggregation of C. albicans WT or ΔALS3 with S. gordonii. (A to F) Fluorescently (FITC) labeled S. gordonii DL1 cells were incubated with C. albicans hypha-forming cells for 1 h. Coaggregated microorganisms were visualized by phase-contrast microscopy (A, C, and E) or by fluorescence microscopy (B, D, and F). Scale bars, 25 μm. Images A to F are representative of samples of S. gordonii DL1 and C. albicans WT exhibiting population heterogeneity with respect to the ability of hyphae to bind bacteria. (G) Percentages of total C. albicans hyphae with attached S. gordonii DL1 determined on the basis of the following binding levels (see Materials and Methods): 2+, hyphae completely surrounded by streptococci (B); 1+, hyphae with areas where streptococcal cells are attached (D); 0, little or no bacterial binding (F). The data are means ± SD from duplicate experiments.

Richard J. Silverman, et al. Infect Immun. 2010 November;78(11):4644-4652.

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