Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 6

1.
Figure 4

Figure 4. Age-dependent loss of NINAC p174 and PKC in retin1. From: Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction.

A, Western blots using head extracts from 7 day-old flies. B, Quantification of the relative protein levels from the Western blots. C, Western blots showing the levels of NINAC and PKC in head extracts from 7 day-old flies. D, Quantification of the relative p174 levels from the Western blots. E, Quantification of the relative PKC levels from the Western blots. F, Quantification of the 80% deactivation times of the light response from 3 day-old flies. G, Quantification of the relative p174 levels from the Western blots from flies of the indicated ages. H, Quantification of the relative PKC levels from 1, 3 and 7 day-old flies. Error bars indicate ±SEM. *, p<0.05, ANOVA, n≥3.

Kartik Venkatachalam, et al. J Neurosci. ;30(34):11337-11345.
2.
Figure 3

Figure 3. Morphology of retin1 photoreceptor cells. From: Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction.

AE, Transmission EMs of sections from the distal regions of ommatidia from wild-type and retin1 compound eyes. Unless indicated otherwise, the flies were reared under a 12 h light/12 h dark cycle. A, 21 day-old wild-type. B, 7 day-old retin1. C, 21 day-old wild-type. D, 21 day-old retin1. E, Dark-reared 21 day-old retin1. F, Average number of rhabdomeres/ommatidium from 15 day-old flies, as quantified using the optical neutralization technique (Franceschini et al., 1981).

Kartik Venkatachalam, et al. J Neurosci. ;30(34):11337-11345.
3.
Figure 2

Figure 2. Loss of Rh1 in retin1 flies. From: Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction.

A, Western blot containing head extracts from 7 day-old wild-type and retin deficient flies probed with Rh1 and Tubulin antibodies. Protein size markers are indicated in kilodaltons. B, Relative Rh1 levels based on Western blots. Error bars indicate ±SEM. *, p<0.05, ANOVA, n≥3. C, Confocal images showing the appearance of ERPs (arrows) in 7 day-old retin1. D, Number of ERPs/rhabdomere. Error bars indicate ±SEM *, p<0.05, Student’s t-test, n≥3. E, PDA in 7 day-old wild-type flies. The lines above the ERGs indicate a 5 second pulses of orange (O) or blue (B) lights. F, Loss of PDA in 7 day-old retin1.

Kartik Venkatachalam, et al. J Neurosci. ;30(34):11337-11345.
4.
Figure 6

Figure 6. Stability of INAD and PKC depends on NINAC p174. From: Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction.

A, Western blot showing the relative levels of INAD in head extracts from 7 day-old wild-type and flies that do not express Retin. The blot was probed with both INAD and Tubulin (Tub) antibodies. B, Quantification of the relative protein levels from blots such as in (A). *, p<0.05, ANOVA, n≥3. C, Western blots of head extracts from 7 day-old wild-type and inaD1 flies probed with PKC, Retin and Tub antibodies. D, Quantification of the relative Retin levels from analyses represented in (C). n.s., not significant. n≥3. E, Western blot of 7 day-old wild-type and ninaCΔ174 head extracts probed with INAD and Tub antibodies. F, Quantification of the relative INAD levels from (E) and duplicate blots. p<0.05, Student’s t-test, n≥3. G, Quantification of the relative INAD levels from Western blots using 1 day-old retin1 and ninaCΔ174 flies. H, Western blots of head extracts from 7 day-old wild-type and ninaCI1501E flies probed with NINAC (to common region of p132 and p174), Retin and Tub antibodies. I, Western blots showing the levels of INAD and PKC in head extracts from 7 day-old ninaCI1501E flies. J, Quantification of the relative INAD and PKC levels from the Western blots such as in (I). K, Western blots showing the levels of INAD and PKC in head extracts from 1 day-old ninaCI1501E flies. L, Quantification of the relative INAD and PKC levels from the Western blots such as in (K). *, p<0.05, ANOVA, n≥3. All error bars indicate ±SEM.

Kartik Venkatachalam, et al. J Neurosci. ;30(34):11337-11345.
5.
Figure 1

Figure 1. Loss of the Retin leads to delayed termination. From: Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction.

A, The retin genomic loci in wild-type and retin1. The genes flanking retin, mms19 and CG12163, are indicated. The red triangles indicate the primers used for the PCR analyses in (B). B, PCR confirmation of the homologous recombination in the retin locus. DNA size markers are indicated in kilobases. C, Confocal images showing the expression patterns of Rh1 (green) and Retin (red) in retinal cross-sections. D, Western blots of head extracts probed with Retin and Tubulin (Tub) antibodies. Protein size markers are indicated in kilodaltons. E, ERGs performed in dark-adapted 9 day-old wild-type and retin1 flies. F, Quantification of the 80% deactivation times. Error bars indicate the means ±SEM *, p<0.05, ANOVA, n≥3.

Kartik Venkatachalam, et al. J Neurosci. ;30(34):11337-11345.
6.
Figure 5

Figure 5. Retin and NINAC p174 interact. From: Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction.

A, p174, but not p132, coimmunoprecipitated with Retin. Retin was immunoprecipitated with Retin antibodies and a Western blot was probed with antibodies that recognize both NINAC isoforms. The band between p174 and p132 is a p174 breakdown product, which is frequently observed as a result of the incubations during the immunoprecipitations. B, Retin coimmunoprecipitated with p174 but not p132. The p132 or p174 proteins were immunoprecipitated with antibodies specific to each protein and a Western blot was probed with a Retin antibodies. Inputs in (A and B) represent 5% of the total. C, Western blot showing the Retin levels in head extracts from 7 day-old flies. D, Western blots showing the levels of Retin in head extracts from 30 minute- and 1 day-old flies. E, Western blot showing the levels of Retin in head extracts. F, Western blots showing the levels of PKC and Rh1 in head extracts from 7 day-old flies. Tubulin (Tub) was used as a loading control in all panels. G, Quantification of the relative PKC and Rh1 levels from Western blots. Error bars indicate SEM. * p<0.05, ANOVA, n≥3.

Kartik Venkatachalam, et al. J Neurosci. ;30(34):11337-11345.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk