Results: 3

1.
Figure 2.

Figure 2. From: Development of a Multiplexed Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Assay to Identify Common Members of the Subgenera Culex (Culex) and Culex (Phenacomyia) in Guatemala.

Ethidium bromide-stained agarose gel showing differentiation of Culex nigripalpus from Cx. thriambus by restriction fragment length polymorphism. Culex thriambus contains a NcoI restriction site in the 207-bp amplicon, whereas Cx. nigripalpus lacks this site. Lane 1: Cx. nigripalpus (digested), Lane 2: Cx. nigripalpus (undigested), Lane 3: Cx. thriambus (digested), Lane 4: Cx. thriambus (undigested), Lane 5: negative control.

Rebekah J. Kent, et al. Am J Trop Med Hyg. 2010 August 5;83(2):285-291.
2.
Figure 1.

Figure 1. From: Development of a Multiplexed Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Assay to Identify Common Members of the Subgenera Culex (Culex) and Culex (Phenacomyia) in Guatemala.

Ethidium bromide-stained agarose gel depicting differentiation of six species of Culex in Guatemala. Lane 1: Cx. (Cux.) quinquefasciatus (580 bp), Lane 2: Cx. (Phc.) lactator (446 bp), Lane 3: Cx. (Cux.) interrogator (354 bp), Lane 4: Cx. (Cux.) coronator (244 bp), Lane 5: Cx. (Cux.) nigripalpus (207 bp), Lane 6: Cx. (Cux.) chidesteri (180 bp), Lane 7: Negative control.

Rebekah J. Kent, et al. Am J Trop Med Hyg. 2010 August 5;83(2):285-291.
3.
Figure 3.

Figure 3. From: Development of a Multiplexed Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Assay to Identify Common Members of the Subgenera Culex (Culex) and Culex (Phenacomyia) in Guatemala.

Ethidium bromide-stained agarose gel showing disproportionate ratios of Culex quinquefasciatus and Cx. interrogator mixed templates, simulating contaminated pools. A fixed volume of Cx. quinquefasciatus template was mixed with serial dilutions of Cx. interrogator template. Lane 1: Cx. quinquefasciatus, Lane 2: Cx. interrogator, Lane 3: equal proportions of both templates 1:1, Lane 4: 1:4, Lane 5: 1:10, Lane 6: 1:20, Lane 7: 1:40, Lane 8: 1:80, Lane 9: 1:160, Lane 10: 1:320, Lane 11: negative control.

Rebekah J. Kent, et al. Am J Trop Med Hyg. 2010 August 5;83(2):285-291.

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